Invitrogen™ Mouse Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) Recombinant Protein

Description

Mouse Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) Recombinant Protein, RMGMCSF20, from Invitrogen.

The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13.
Host Species: E. Coli
Formulation: 20μg purified protein and no preservative
Purity: >98% pure by SDS-PAGE and RP-HPLC analyses. Activity: One unit of activity is <0.05 ng/ml, as determined in a proliferation assay of mouse FDC-P1 cells, corresponding to a specific activity of >2x10^7 units/mg. Use: Mouse GM-CSF exerts activity in the range of 0.05 to 0.5 ng/ml for most in vitro applications. Reconstitution and Dilution: Reconstitute in distilled water to a concentration of 100 ug/ml. After reconstitution, dilute cytokine in a neutral buffer containing carrier protein such as 5-10% FBS or 1-4% high purity BSA (fatty acid free fraction V). Lyophilized product is stable until expiration if stored desiccated below 0ºC. Cytokines diluted as indicated above should be divided into aliquots and stored at -70ºC. Recombinant proteins should be stored at concentrations >500 ng/ml to reduce loss of activity. This product contains 124 amino acid residues. Endotoxin level is <1 EU/ug.