Cytiva

Cytiva

Welcome to Cytiva Lab Recharge 2021

Offering solutions for all segments and applications in life sciences research including sample preparation, protein purification, cell culture, filtration and Western blotting.
Contact your local Fisher Scientific Representative and ask for your promo code to unlock your special prices.

Click on the workflow for more products




Protein Research

Choose from Cytiva's portfolio of protein purification tools, from Sephadex™, the chromatography resin that revolutionised protein purification, to the newest magnetic bead-based technology. Cytiva's products support a broad range of chromatography resins and resin methods, including affinity purification, gel filtration, desalting, ion exchange and hydrophobic interaction, all in formats to meet your application requirements.

  • Loose resin up to 500g
  • Chromatography columns such as HiTrap™, SpinTrap™, GraviTrap™ and Tricorn™ columns
  • Pre-packed columns for convenience and efficiency

Western Blotting

Amersham™ Western blotting products deliver reliable protein identification for you time after time: meaningful data, reproducible results.


Key Products

Cell Culture

HyClone™ serum, media buffers and process liquids have helped advance drug discovery and bioproduction processes since 1967, and continue to deliver quality and consistency.

HyClone™ Classical and Serum-Free Media »

Culture cells using our complete line of HyClone™ classical, speciality and transfection media products that support cell growth of CHO, SF9, HEK293, hybridoma, myeloma, insect, NS0 and viral vaccine cell lines.

HyClone™ Fetal Bovine Serum (FBS), Calf Serum, Fetal Bovine Serum Alternatives, and Supplements »

Enhance cell culture performance using a variety of HyClone™ supplements, including Fetal Bovine Serum (FBS), calf serum, engineered serum (FetalClone™ 1, 2, 3) and serum-free supplements (HyClone™ Cell Boost 1-6).

HyClone™ Antibiotics and Reagents »

Separate attachment-dependent cells from substrate or reduce the effect of bacteria, mould or yeast contaminants in your cell culture process.

HyClone™ Stem Cell Products »

Grow stem cells with ease using our specialty line of stem cell products tailored to optimise cell culture performance for a variety of stem cells, including induced pluripotent stem cells (iPSC), mesenchymal stem cells (mSC), human embryonic stem cells (hESC) and epithelial stem cells.

HyClone™ Buffers and Process Liquids »

Manage, control and regulate pH, temperature, osmotic pressure, carbon dioxide and oxygen for your specific cell culture.

11638_Cell_Culture_Images2

Key Products

DNA and RNA Research

When nucleic acid (DNA or RNA) sample is scarce, amplification can be used to expand the sample quantity for use in multiple experiments. Selecting the most suitable amplification technology will depend on sample type, the molecular biology application where it will be used, and the need to preserve sample for additional testing. Some common technologies for DNA amplification are Phi29 polymerase technology, polymerase chain reaction (PCR), rolling circle amplification (RCA), and isothermal multiple strand displacement amplification (MDA).

Phi29 polymerase technology

Phi29 DNA polymerase-based isothermal DNA amplification is a simple, reliable alternative to other DNA amplification procedures such as polymerase chain reaction (PCR). The highly processive Phi29 DNA polymerase elicits strong strand displacement enabling rapid DNA replication from multiple sites. 

Whole genome amplification

GenomiPhi™ products, powered by Phi29 polymerase technology, are available in kits that provide an easy-to-use method which delivers highly representative and reliable whole genome amplification. Each kit contains all the components necessary for whole-genome amplification by utilising isothermal multiple strand displacement amplification, with minimal hands-on time. Compared with conventional PCR amplification techniques, MDA generates larger sized products with a lower error frequency.

Rolling circle amplification

TempliPhi™, based on Phi29 DNA polymerase, is used to exponentially amplify single- or double-stranded circular DNA by rolling circle amplification (RCA). This isothermal amplification produces microgram quantities of DNA from picograms of starting material in 4 to 6 hours.


Whatman™ Filtration

Filtration is an indispensable step in sample preparation and many analytical methods. Cytiva's Whatman™ products provide the quality, consistency, reliability and convenience to help your lab run smoothly. With a large variety of available sizes, types and specifications, you can be sure to find a filter that fits your application, equipment and workflow.

  • Cellulose, glass and quartz fibre filters
  • Membrane filters
  • Syringe and syringeless filtration devices
  • Capsule filters
  • Sterile and non-sterile options
  • Lab essentials

14744_GE_HPLC_Landing_Banner

Whatman Puradisc syringe filter

  • High performance, dependable single membrane layer filter
  • Available in 11 different membrane types and seven pore sizes to work for samples of many chemical compositions and volumes

Whatman GD/X™ syringe filter

  • Used for heavy, thick, or viscous hard-to-filter samples
  • Multi-gradient glass-fibre layer pre-filters large particles which increases flow rate and volume through the main membrane

Whatman GD/XP syringe filter

  • Used for heavy, thick, or viscous samples with low protein binding and low metals extractables
  • Polypropylene pre-filtration stack provides increased flow rate and volume flow through while eliminating protein binding and inorganic ion extractables that could come from glass fibre pre-filtration 

Whatman SPARTAN™ certified

  • Every batch is tested and certified for HPLC use to ensure batch-to-batch consistency and low levels of UV-absorbing substances
  • Regenerated cellulose membrane exhibits very low protein binding and low levels of extractables in water, methanol and acetonitrile

Whatman Anotop™

  • Recommended for use in ion chromatography 
  • Alumina-based Anopore membrane has very low ion extractables and highly uniform capillary pore structure

Whatman Mini-UniPrep™ (MUP)

  • Designed for HPLC and UHPLC autosamplers to support high throughput testing labs
  • Combines syringe, syringe filter, autosampler vial, cap and septum in a single device for increased efficiency in consumable management

Whatman Mini-UniPrep™ (MUP) G2

  • Designed for HPLC and UHPLC autosamplers to support high throughput testing labs requiring low levels of organic leachables
  • Same design as MUP but a borosilicate glass vial reduces leachables that could come from plastics

Cellulose fibre - quantitative

  • Low ash filters in support gravimetric and other weight-based analyses
  • Available in a variety of thicknesses, retention sizes and surface treatments to support specific applications

Cellulose fibre - qualitative

  • High purity cotton filters support sample separation, clarification and purification in advance of analysis
  • Available in a variety of thicknesses, retention sizes and physical preparations to support specific applications

Glass and quartz fibre

  • The ability to capture very small particles supports environmental analysis and other applications requiring high retention rates
  • Available with a range of particle retention sizes, mechanical strengths and thermal stabilities to support specific applications


Key Products

Syringe Filters

  • Whatman Puradisc
  • Whatman GD/X
  • Whatman GD/XP
  • Whatman SPARTAN certified
  • Whatman Anotop
  • Whatman Mini-UniPrep (MUP) and G2
11638_syringe_filters

Filter papers, and glass/quartz fibres

  • Cellulose fibre – quantitative
  • Cellulose fibre – qualitative
  • Glass and quartz fibre
11638_filter_papers
Resources

More


Products
Cytiva Thrombin Protease

Cytiva Thrombin Protease

For site-specific separation of the GST tag from proteins expressed using pGEX-T vectors THROMBIN PROTEASE

Cytiva HyClone™ Penicillin Streptomycin 100X Solution

Cytiva HyClone™ Penicillin Streptomycin 100X Solution

Used to control/destroy destructive microorganisms such as bacteria 100ML PEN STREP SOLUTION

Cytiva Disposable PD-10 Desalting Columns

Cytiva Disposable PD-10 Desalting Columns

Use for desalting and buffer exchange, as well as removal of low-molecular weight compounds. Cytiva Life Sciences™ Disposable PD-10 Desalting Columns provide fast, high-recovery. X30 Disposable PD-10 desalting column

Cytiva HyClone™ Fetal Bovine Serum, South American Origin

Cytiva HyClone™ Fetal Bovine Serum, South American Origin

500ML FETAL BOVINE SERUM, Research GradeSouth American Origin, European Customers

Cytiva PEEK Tubing

Cytiva PEEK Tubing

PEEK tubing comes in a variety of internal diameter sizes.  Cytiva Lifescience™ PEEK Tubing is an important chromatography accessory. TUBING PEEK 0.15MM I.D., 1/16 INCH O.D., 2M ACCESSory for Ettan(TM) LC

Cytiva HyClone™ Trypsin Protease

Cytiva HyClone™ Trypsin Protease

Derived from porcine pancreas. HyClone™ Trypsin Protease is used to disaggregate adherent cells from substrate or surface to which they are attached. 100 ML TRYPSIN 0.25% (1X) SOLUTION, WITH 0.1%EDTA Hyclone 100mL Store in Freezer at -20C

Cytiva L-Glutamine

Cytiva L-Glutamine

Supplement serum- and protein-free media using this amino acid. 500ML L-GLUTAMINE 200 MM85% NaCl (Flüssigkeit), 500ml

Cytiva HyClone™ Dulbecco's Phosphate Buffered Saline, Solution

Cytiva HyClone™ Dulbecco's Phosphate Buffered Saline, Solution

Buffer cell culture with PBS in solution. DPBS 1X -CA MG PR 100ML

Cytiva Amersham™ ECL Select™ Western Blotting Detection Reagent

Cytiva Amersham™ ECL Select™ Western Blotting Detection Reagent

Use as a high sensitivity reagent for chemiluminescent Western blotting detection. Cytiva Life Sciences™ Amersham™ ECL Select™ Western Blotting Detection Reagent  provides highly intense signal output, for detection of medium to very low protein levels. WB DETECTION REAGENT KIT, ECL SELECT, HIGH SENSITIvity chemiluminescent substrate for1000cm2 of

Cytiva illustra™ NAP™ Columns, NAP-5

Cytiva illustra™ NAP™ Columns, NAP-5

Use for rapid and efficient purification of DNA and oligonucleotides (>10-mer) in less than 15 minutes by gravity flow. Cytiva Life Sciences™ illustra™ NAP™ Columns are disposable columns prepacked with Sephadex™ G-25 DNA Grade X20 illustra NAP-5 columns 00

Cytiva Ettan™ IPGphor™ 3 Isoelectric Focusing System

Cytiva Ettan™ IPGphor™ 3 Isoelectric Focusing System

Fully integrated equipment optimized to deliver high throughput, speed, reproducibility and high protein-loading capacity. ISOELECTRIC FOCUSING UNIT ETTAN IPGPHOR FOR 2Dgel electrophoresis includes cable, software, and

Cytiva Plastic TC Clamp

Cytiva Plastic TC Clamp

For use with the ÄKTA™ Pilot chromatography system. Cytiva Plastic TC Clamp is a white 25mm tri-clamp for providing a sanitary, secure connection. X4 CLAMP ATKAPILOT 25MM (PACK OF 4)

Cytiva ÄKTA™ Pilot Column Tubing Kit

Cytiva ÄKTA™ Pilot Column Tubing Kit

Sanitarily designed to prevent leakage. Cytiva ÄKTA™ Pilot Column Tubing Kit includes 5/16 in. connectors, gaskets, O-rings, and S8 tubing for use in the column valve of the ÄKTA™ Pilot chromatography system. COLUMN TUBING KIT AKTAPILOT, CONTAINS ALL TUBINGand connectors necessary to install 2 x columns

Cytiva Hyclone™ Characterized Fetal Bovine Serum (Australia)

Cytiva Hyclone™ Characterized Fetal Bovine Serum (Australia)

Characterized FBS irradiated is comparable to the highest quality FBS from other suppliers, but is lower priced than defined FBS. FBSCHAR Australian New Zealand Filtered 1000 ML IRR

Cytiva EPS 301 Electrophoresis Power Supply

Cytiva EPS 301 Electrophoresis Power Supply

Cover the wide range of electrophoresis and blotting applications. Designed for performance, EPS Power Supplies can be relied on for security and reproducibility POWER SUPPLY EPS 301 120-240V 50-60H z 5 to 300V,80W max

Cytiva Sample Loop Kit

Cytiva Sample Loop Kit

Set of 5 loops for loading/injecting samples onto a chromatography column. Cytiva Sample Loop Kit is a set of small PTFE loops (25, 50, 100, 200, 500μL capacities) for use with autosampler/injections valves (e.g. Valve V-7) and ÄKTA™ Chromatography Systems. SAMPLE LOOP PACK ASSORTED PTFE 25µL, 50µL, 100µL,200µL, 500µL

Cytiva Tubing Connector for Use with Peristaltic Pump P-1

Cytiva Tubing Connector for Use with Peristaltic Pump P-1

Used for the connection of 1.8mmOD/1.5mmID capillary tubing with the Peristatic Pump P-1. X6 Pump P-1 Tubing Connector

Cytiva PEEK Union

Cytiva PEEK Union

Connect piping of varying sizes. Cytiva Life Sciences™ PEEK Unions are an important accessory for various scientific applications. X5 UNION M6 FEMALE/1/16 INCH MALE (PACK OF 5)

Cytiva NHS-Activated Sepharose™ 4 Fast Flow Affinity Media

Cytiva NHS-Activated Sepharose™ 4 Fast Flow Affinity Media

Use for coupling amino-containing smaller proteins and peptides.  Cytiva Life Sciences™ NHS-Activated Sepharose™ 4 Fast Flow Affinity Media yields coupled products that are very stable, especially at high pH. 25 ML NHS-activated Sepharose(TM) 4 Fast Flow,

Cytiva Protein and Peptide Molecular Weight Markers

Cytiva Protein and Peptide Molecular Weight Markers

Run these markers to determine the molecular weight of uncharacterized protein samples with precision. Cytiva Life Sciences™ Protein and Peptide Molecular Weight Markers work by comparing unknowns with carefully selected standards. CALIBRATION KIT HIGH MOLECULAR WEIGHT (SDS) FORElectrophoresis (53-220KDa) 00Store in Fridge at

Cytiva Streptavidin Sepharose™ High Performance Medium

Cytiva Streptavidin Sepharose™ High Performance Medium

Used for convenient, fast, reliable purification of biotinylated compounds. Streptavidin Sepharose™ High Performance Medium is a physically and chemically stable medium. STREPTAVIDIN SEPHAROSE HP,5 ML

Cytiva Cy3 NHS Ester Mono-Reactive CyDye

Cytiva Cy3 NHS Ester Mono-Reactive CyDye

Use for highly accurate labeling of amine groups. Cytiva Cy3 NHS Ester Mono-Reactive CyDye contain derivitized CyDyes that carry only one reactive group on each dye molecule for accurate labeling of amine groups, for example on antibodies, peptides, or oligonucleotides. CY3 MONO NHS ESTER 5MG

Cytiva M6 Male Tubing Connectors

Cytiva M6 Male Tubing Connectors

M6 tubing connectors constructed of durable plastic materials for use in chromatography pump systems. Cytiva M6 Tubing Connectors are available in male flanged, flangeless and end fitting connections. X2 TUBING CONNECTOR FLANGELESS M6 MALE FOR HITRAP(TM) column (pack of 2)

Cytiva Whatman™ Binder-Free Glass Microfiber Filters GF/F Circles

Cytiva Whatman™ Binder-Free Glass Microfiber Filters GF/F Circles

Served as the basis filter for EPA standard for the toxicity characteristic leaching procedure. Whatman™ Binder-Free Glass Microfiber Filters GF/F Circles offers an extremely high loading capacity and flow rate. X100 GF/F 15MM

Cytiva Injection Needle

Cytiva Injection Needle

With O.D. 0.7mm guidance X6 Injection Needle o.d. 0.7mm

Cytiva HyClone™ RPMI 1640 Media

Cytiva HyClone™ RPMI 1640 Media

Designed to support cell culture growth and biomanufacturing processes. RPMI 1640 1X +GLUT 6X1000MLSH30091), 6 X1000 mL

Cytiva Glutathione Sepharose™ 4B Media

Cytiva Glutathione Sepharose™ 4B Media

Use for high capacity purification of Glutathione S-Transferase (GST) tagged fusion proteins. Cytiva Life Sciences™ Glutathione Sepharose™ 4B Media requires only a single-step for purification. GLUTATHIONE SEPHAROSE 4B, 300 ML

Cytiva illustra™ MicroSpin™ G-25 Columns

Cytiva illustra™ MicroSpin™ G-25 Columns

Use for the rapid purification of DNA >10 base pairs in length in a wide range of applications. Cytiva Life Sciences™ llustra™ MicroSpin™ G-25 Columns are ideal for desalting, buffer exchange, and removal of unincorporated nucleotides from end-labeled oligonucleotides.  X50 MICROSPIN G-25 columns 00

Cytiva Inlet filter holder kit

Cytiva Inlet filter holder kit

Use as filter holder for buffer inlet tubes.   Cytiva Lifescience™ Inlet filter holder kit is an important chromatography accessory. FILTER HOLDER KIT

Cytiva Streptavidin HP MultiTrap™ Plates

Cytiva Streptavidin HP MultiTrap™ Plates

Fast, reliable protein enrichment through immobilized biotinylated biomolecules in prepacked 96-well filter plates X4 STREPTAVIDIN HP MULTITRAP

  spinner

ÄKTA™ Quote/ Info Request
GEAkta

ÄKTA™ start protein purification system

You won’t believe how easy it is.

From method creation and sample loading to fraction collection and data analysis, we’ve thought about how to make your work easier at every step. Let ÄKTA start transform your manual purification workflow into a convenient automated solution that delivers reproducible results.

It’s like having an expert with you all the way.

UNICORN start system control software is user-friendly, guiding you through each step in your purification, from creating a protocol and setting up your run to evaluating your results and creating a custom report. See how UNICORN start software saves you time and gives you valuable insight into your run or purification.

ÄKTA start offers the following features:

  • Compact solution for quick and reliable one-step protein purification
  • Sample injection and fraction collection options
  • Quick start methods and templates for common purification techniques
  • Intuitive touchscreen display and real-time monitoring

Specifications:

  • Flow rate: 0.5 to 5mL/min
  • Pump type: peristaltic pump - single channel, four-roller pump head with low pulsation
  • Max. operating pressure: 0.5MPa
  • UV, fixed wavelength: Yes, 280nm
  • Conductivity range: 0 to 300mS/cm
  • Software: UNICORN start 1.0 or higher
Cytiva logo

Cytiva: speed, efficiency, capacity

Cytiva is a global provider of technologies and services that help advance and accelerate the development and manufacture of therapeutics. With a heritage of innovation, we continue to move forward, building on the brands you know and have come to rely on for quality and reliability. Our diverse portfolio includes well-recognised brands such as ÄKTA™, Amersham™, HyClone™, MabSelect™ and Whatman™. Cytiva brings speed, efficiency and capacity to life sciences research and manufacturing, enabling the delivery of transformative medicines
to patients.