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Thermo Scientific™ CloneJET PCR Cloning Kit
Efficiently clone PCR products generated with any thermostable DNA polymerase using Thermo Scientific™ CloneJET™ PCR Cloning Kit, a positive selection system.
Antibiotic Resistance Bacterial | Ampicillin (AmpR) |
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Format | Kit |
Sample Type | PCR Products |
Cloning Method | Restriction Enzyme⁄ MCS, Blunt PCR |
For Use With (Application) | PCR Cloning |
Vector | pJET1.2/blunt |
Product Line | CloneJET |
Thermo Scientific™ Lambda DNA
Generate DNA size marker fragments with this linear, double-stranded DNA, a common substrate for restriction endonucleases.
Invitrogen™ GeneArt™ Gibson Assembly HiFi Master Mix
GeneArt Gibson Assembly HiFi Master Mix enables DNA assembly and cloning via a technique that allows multiple overlapping DNA fragments to be seamlessly linked in a one-step, 15–60 minute isothermal reaction.
Shipping Condition | Dry Ice |
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Content And Storage | Includes master mix, positive control, and water and accommodates the use of your own competent cells. |
Workflow Step | DNA Assembly |
Number of Fragments | Up to 6 fragments |
Product Type | HiFi Master Mix |
Control Type | Positive Control |
Cloning Method | Seamless cloning |
For Use With (Application) | Seamless Cloning & Genetic Assembly |
Product Line | GENEART™ |
Thermo Scientific™ pUC19 DNA
Optimize cloning with this double-stranded closed circular high copy plasmid DNA, 2686 base pairs with a molecular weight of 1.74 x 106 Da isolated from E.coli.
Thermo Scientific™ Rapid DNA Ligation Kit
Enable fast sticky-end or blunt-end DNA ligation in only 5 minutes at room temperature.
Product Type | Rapid DNA Ligation Kit |
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Enzyme | DNA Ligase |
Thermo Scientific™ pBR322 Plasmid DNA
Optimize cloning with Thermo Scientific™ pBR322 DNA, a 4361 bp double-stranded closed circular medium copy plasmid DNA.
Invitrogen™ Second Strand cDNA Synthesis Kit
Invitrogen™ Second Strand cDNA Synthesis Kit is design to produce double-stranded cDNA using first strand reaction without the need for intermediate organic extraction or ethanol precipitation steps. This convenient single-tube format speeds up the synthesis procedure and maximizes cDNA recovery. The kit contains premixed components to reduce the number of pipetting steps necessary to complete the procedure.
Reaction Format | Separate Components |
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Final Product Type | Double Stranded cDNA |
Product Type | cDNA Synthesis Kit |
For Use With (Application) | Cloning |
Applied Biosystems™ BigDye™ Terminator v3.1 Cycle Sequencing Kit
The BigDye™ Terminator v3.1 Cycle Sequencing Kit's robust, highly flexible chemistry
Product Type | Sequencing Buffer |
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For Use With (Equipment) | 310 Genetic Analyzer, 3130xl Genetic Analyzer, 3730xl DNA Analyzer, GeneAmp 9700, Veriti Thermal Cycler, 3130 Genetic Analyzer, 3730 DNA Analyzer, 3500xL Genetic Analyzer, 3500 Genetic Analyzer |
Invitrogen™ GeneArt™ Gibson Assembly EX Master Mix
GeneArt Gibson Assembly EX Master Mix enables DNA assembly via a technique that allows multiple overlapping DNA fragments to be seamlessly linked in a single-tube, two-step optimized reaction.
Shipping Condition | Dry Ice |
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Content And Storage | Includes master mix, positive control, and water and accommodates the use of your own competent cells. |
Workflow Step | DNA Assembly |
Number of Fragments | Up to 15 fragments |
Product Type | Master Mix |
Control Type | Positive Control |
Cloning Method | Seamless cloning |
For Use With (Application) | Seamless Cloning & Genetic Assembly |
Product Line | GENEART™ |
Invitrogen™ GeneRacer™ Kit with SuperScript™ III RT and TOPO TA Cloning™ Kit for Sequencing
Ensures amplification of only full-length transcripts via elimination of truncated messages from amplification process
Applied Biosystems™ POP-7™ Polymer for 3730/3730xl DNA Analyzers
POP-7™ Polymer is a separation matrix for the 3730 and 3730xl DNA Analyzers. POP-7™ Polymer is customized for DNA sequencing and fragment analysis.
Accuracy | 98.5% up to 900 bases (for 50 cm capillary), 98.5% up to 700 bases (for 36 cm capillary) |
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Product Type | Polymer |
For Use With (Application) | Standard Sequencing, Fragment Analysis (Denaturing) |
Test Time | 1 hr for 36 cm capillary (std. seq.), 2 hrs for 50 cm capillary (long read seq.) |
Product Line | POP-7™ |
Thermo Scientific™ Phusion™ Site-Directed Mutagenesis Kit with DH10B Competent Cells
A versatile and efficient tool for introducing point mutations, insertions, or deletions into any type of plasmid DNA. With this kit the entire plasmid is amplified using phosphorylated primers that introduce the desired changes.
Invitrogen™ TA Cloning™ Kit, with pCR™2.1 Vector and One Shot™ INVαF' Chemically Competent E. coli
Quick, one-step cloning strategy for directly inserting Taq-amplified PCR product into plasmid vector
Promoter | T7 |
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Content And Storage | TA Cloning™ kits contain linearized pCR™2.1 vector, ExpressLink™ T4 DNA ligase, 5X ExpressLink™ T4 DNA ligation buffer, dNTPs, 10X PCR buffer, sterile water, and controls. The competent cell kits contain One Shot™ chemically competent E. coli, S.O.C. medium, and a supercoiled control plasmid. Store One Shot™ E. coli at -80°C. Store all other components at -20°C. All reagents are guaranteed stable for 6 months when properly stored. |
Format | Kit |
Product Type | Cloning Kit |
Cloning Method | TA Cloning |
Bacterial or Yeast Strain | INVαF ́ |
For Use With (Application) | PCR Cloning |
Product Line | One Shot™ |
Cell Type | Chemically Competent E. coli |
Thermo Scientific™ Maxima H Minus Double-Stranded cDNA Synthesis Kit
The Thermo Scientific Maxima H Minus Double-Stranded cDNA Synthesis Kit is a complete system for efficient synthesis of double-stranded cDNA from total RNA or mRNA.
Reverse Transcriptase | Maxima H Minus |
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Reaction Format | Separate Components |
Final Product Type | Double Stranded cDNA |
Product Type | cDNA Synthesis Kit |
Sample Type | RNA |
For Use With (Application) | Cloning |
Product Line | Maxima |