PCR Specific Buffers

Invitrogen™ UltraPure™ Phenol:Chloroform:Isoamyl Alcohol (25:24:1, v/v)

Nucleaic acid purfication PHENOL/CHLOROFORM/ALCOHOL

Invitrogen™ Nuclease-Free Water (not DEPC-Treated)

4LT NUCLEASE-FREE WATER 4X1 LITER

Invitrogen™ UltraPure™ Glycerol

Low temperature enzyme storage and concentration 3.8 LT GLYCEROL REDISTILLED

Invitrogen™ EDTA (0.5M), pH 8.0

Ready-to-use, certified RNase-free 1 LT 0.5 M EDTA, PH 8.0 1L STORE AT ROOM TEMPERATUre

Invitrogen™ AccuPrime™ Pfx SuperMix

High-fidelity, high-specificity amplification of DNA fragments 200 RXN PFX SUPERMIX 200 REACTIONS STORE AT -20C

Invitrogen™ PBS Phosphate-Buffered Saline (10X) pH 7.4

Dilute to desired concentration 10X PBS BUFFER, PH 7.4 500 MLMolecular biology grade, Ambion® PBS 10X, pH 7.4

Invitrogen™ Salmon Sperm DNA, sheared (10 mg/mL)

Blocks non-specific hybridization SALMON SPERM DNA (SHARED)10 tubes

Invitrogen™ Acid-Phenol:Chloroform, pH 4.5 (with IAA, 125:24:1)

Premixed, safer, and easier alternative to preparing crystalline phenol solutions 400 ML ACID-PHENOL:CHLOROFORM, PH 4.5 (WITH IAA,125:24:1) 400mL Store at 4°C

Thermo Scientific™ Phusion Buffers

Choose between two buffer packs to optimize high-fidelity PCR or difficult or long template amplification using Thermo Scientific™ Phusion™ High-Fidelity DNA Polymerases. DETERGENT-FREE GC BUFFER 6 MLML

Invitrogen™ RPA III™ Ribonuclease Protection Assay Kit

Fast single-tube reaction RPA III KIT 35 RXNreactions

Invitrogen™ TE, pH 8.0

Ready-to-use, certified RNase-free 500 ML TE, PH 8.0 500ML STORE AT ROOM TEMPERATURE

Invitrogen™ RT-PCR Grade Water, Not DEPC-treated

RT-PCR GRADE WATER 10 X 1.7MLAmbion® RT-PCR grade water is tested for

Invitrogen™ Linear Acrylamide (5 mg/mL)

Useful for separating reaction products from unincorporated nucleotides and oligonucleotide primers LINEAR ACRYLAMIDE 5 X 1 MLAmbion® Linear Acrylamide (5 mgmL) is for use as

Invitrogen™ KCl (2M)

Ready-to-use, certified RNase-free 100 ML 2 M KCL 100ML STORE AT ROOM TEMPERATURE

Invitrogen™ UltraPure™ Cesium Chloride, Optical Grade

Highest grade available 1 KG CAESIUM CHLORIDE

Invitrogen™ DEPC-Treated Water

4000ML DEPC-TREATED WATER 4 X 1000 ML

Invitrogen™ THE RNA Storage Solution

Features to minutesimize base hydrolysis of RNA 50 ML THE RNA STORAGE SOLUTION 50ML STORE AT -20 C

Thermo Scientific™ MagJET™ mRNA Enrichment Kit

Purify mRNA from total RNA samples with oligo (dT) poly A+-binding magnetic beads with up to 10-fold enrichment from 5-100 μg RNA input MagJET mRNA Enrichment Kit

Invitrogen™ AccuPrime™ Pfx DNA Polymerase

Provides higher fidelity than Pfu DNA polymerase 1000 RXN PFX DNA POLYMERASE ACCUPRIME(TM) 1000reactions Store at -20C

Invitrogen™ MgCl2 (1M)

Ready-to-use, certified RNase-free 1 M MAGNESIUM CHLORIDE 100MLMgCl2solution, 100 ml

Invitrogen™ UltraPure™ Dithiothreitol

High and low concentration applications DITHIOTHREITOL (DTT)

Invitrogen™ Sodium Acetate (3M), pH 5.5

Ready-to-use, certified RNase-free 100 ML 3 M SODIUM ACETATE, PH 5.5 100ML STORE ATroom temperature

Invitrogen™ GlycoBlue™ Coprecipitant (15mg/mL)

Blue dye increases pellet visibility 15 MG GLYCOBLUE 15MG STORE AT -20°C

Invitrogen™ Formamide (Deionized)

Nuclease-free, deionized 2500GR FORMAMIDE (DEIONIZED) 5 X 500G

Thermo Scientific™ MgCl2 (Magnesium Chloride)

Optimize your PCR reaction conditions with this Magnesium Chloride (MgCl2) solution. 5ML 25MM MGCL2

Invitrogen™ TE, pH 7.0

Nuclease-free, ultrapure solution for RNA samples TE BUFFER 10 X 1.0 MLAmbion® TE buffer is a commonly recommended

Invitrogen™ UltraPure™ RNase A (20mg/mL)

Isolates RNA-free DNA 10 ML RNASE A 10ML SHELF LIFE = 3650 PACKINGinstructions = RRR Storage = Fridge 4C

Invitrogen™ Tris (1M), pH 8.0

Ready-to-use, certified RNase-free 500 ML 1 M TRIS, PH 8.0 500ML STORE AT ROOM TEMPERature

Invitrogen™ Glycogen (5 mg/ml)

Enhances precipitation of nucleic acids GLYCOGEN 5 X 1 MLAmbion® Glycogen is a branched chain carbohydrate

Invitrogen™ Acrylamide/Bis 19:1, 40% (w/v) solution

Ready-to-use solution reduces acrylamide and bis-acrylamide powder contact hazards 500 ML ACRYLAMIDE BIS 19:1 40% SOLUTION 500MLStore at 4°C

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