Invitrogen™ 25-Hydroxy Vitamin D3 Competitive ELISA Kit

ELISA

The 25 Hydroxy Vitamin D3 (25 HVD3) ELISA quantitates 25 HVD3 in serum, plasma and other biological fluids. Principle of the method The competitive multispecies ELISA research use only kit is designed to quantitatively measure 25-Hydroxy Vitamin D3 (25-HVD3), independent of species . A 25-HVD3 standard is provided to generate a standard curve for the assay and all samples are read off a user generated standard curve. Standards or diluted samples are pipetted into a coated microtiter plate and Biotinylated Detection Ab specific to 25-Hydroxy Vitamin D3 is added. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each well and incubated. A TMB substrate solution is then added to each well. The enzyme substrate reaction is terminated by the addition of stop solution and the color change is measured on a microplate reader. The concentration of 25-HVD3 in the samples is then determined by comparing the OD of the samples to the standard curve. Rigorous validation: Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation. For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.