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ACVR2A Human Protein

ACVR2A Human Protein

Brand:  Invitrogen PR9111A

Additional Details : Weight : 0.01000kg

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Product Code. 15458713

  • 12870.00 SEK / 100µg


Estimated Shipment date
from Supplier 28-12-2017
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Disclaimers:

For Research Use Only. Not for use in diagnostic procedures.


Description

Description

This recombinant fusion protein consists of full-length activin A receptor kinase and a glutathione S-transferase (GST) tag. It is validated in the LanthaScreen™ Eu Kinase Binding Assay. No special measures were taken to activate this kinase.
  • Compatible and validated with the LanthaScreen™ Eu Kinase Binding Assay
  • Expressed in insect cells
  • GST tagged for use with Eu-labeled GST antibody

    Easily Screen for Kinase Binding
    The LanthaScreen™ Eu Kinase Binding Assay is a TR-FRET assay that lets you measure inhibitor binding to non-activated kinases, perform simple off-rate measurements, and makes simple the study of type III (allosteric) inhibitors.

    The LanthaScreen™ Eu Kinase Binding Assay is easy to optimize and run. The assay uses a simple mix-and-read format with a single one hour incubation and no development steps. Simultaneous binding of an Alexa Fluor™ conjugate or “tracer” to a tagged kinase is detected by addition of a europium-labeled anti-tag antibody, in this case anti-GST. Simultaneous binding of the tracer and antibody to the kinase results in a high degree of fluorescence resonance energy transfer (FRET), while displacement of the tracer with a kinase inhibitor results in a loss of FRET (Fig 1).

    ACVR2A for the LanthaScreen Kinase Binding Assay
    Activin A receptor kinase (ACVR2A) is a type II member of the TGF-beta family of receptor Serine/Threonine kinases. ACVR2A is involved in the activin and myostatin signaling pathway. (ACVR2A Aliases: ACVR2, ACTRII).

    This insect cell-expressed recombinant fusion protein of full-length ACVR2A and GST tag has been validated in the LanthaScreen™ Eu Kinase Binding Assay with these reagents:
  •  Tracer 236
  •  Eu-labeled GST Antibody

    For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
Specifications

Specifications

NP001607.1
Protein assays and analysis
Frozen
ACVR2A Human Protein
100μg
For research use only. Not for use in diagnostic procedures
ACVR2A, ACTR2, ACTRII, ACVR2
ACVR2A was pre-diluted in enzyme dilution buffer and assayed in 20mM Tris (pH 7.5), 10 MnCl2, 1mM EGTA, 1mM Na3VO4, 5XmM β-glycerophosphate, 2mM DTT, 0.02% Triton™ X-100, 100μM ATP, 300μg/mL dephosphorylated casein substrate and trace [32P]-γ-ATP for 10 minutes at 30°C
Baculovirus
ACVR2A Human Protein
Human
0.44mg/mL
Frozen
85.3kDa
75% by SDS-PAGE
–80°C
1 Each
ACVR2A Human Protein
Kinase assay
STKR
24nmoles of phosphate transferred to dephosphorylated casein substrate per minute per mg of total protein at 30°C. Activity determined at a final protein concentration of 3.33μg/mL
GST Tag
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