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Invitrogen™ ACVR2A Human Protein
Easily screen for kinase binding. Thermo Fisher Invitrogen™ ACVR2A Human Protein recombinant fusion protein consists of full-length activin A receptor kinase and a glutathione S-transferase (GST) tag. It is validated in the LanthaScreen™ Eu Kinase Binding Assay.
Brand: Invitrogen™ PR9111A
Additional Details : Weight : 0.01000kg
Description
The LanthaScreen™ Eu Kinase Binding Assay is a TR-FRET assay that lets you measure inhibitor binding to non-activated kinases, perform simple off-rate measurements and makes simple the study of type III (allosteric) inhibitors.
The LanthatScreen™ Eu Kinase Binding Assay is easy to optimize and run. The assay uses a simple mix-and-read format with a single one-hour incubation and no development steps. Simultaneous binding of an Alexa Fluor™ conjugate or tracer” to a tagged kinase is detected by addition of a europium-labeled anti-tag antibody, in this case anti-GST. Simultaneous binding of the tracer and antibody to the kinase results in a high degree of fluorescence resonance energy transfer (FRET), while displacement of the tracer with a kinase inhibitor results in a loss of FRET.
- Compatible and validated with the LanthaScreen™ Eu Kinase Binding Assay
- Expressed in insect cells
- GST tagged for use with Eu-labeled GST antibody
- No special measures were taken to activate this kinase
ACVR2A for the LanthaScreen Kinase Binding Assay
Activin A receptor kinase (ACVR2A) is a type II member of the TGF-beta family of receptor Serine/Threonine kinases. ACVR2A is involved in the activin and myostatin signaling pathway. (ACVR2A Aliases: ACVR2, ACTRII).
This insect cell-expressed recombinant fusion protein of full-length ACVR2A and GST tag has been validated in the LanthaScreen™ Eu Kinase Binding Assay with the Tracer 236 and Eu-labeled GST reagents.
Specifications
NP001607.1 | |
50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.5mM EDTA, 0.02% NP-40, 2mM DTT and 50% glycerol. | |
100μg | |
RUO - research use only | |
ACVR2A, ACTR2, ACTRII, ACVR2 | |
Human | |
GST Tag | |
Frozen |
Enzyme and Protein Activity Assay, Kinase Assay, Protein Assays and Analysis, Protein Biology | |
85.3kDa | |
Store at -80°C. At first use, aliquot and store at -80°C to avoid multiple freeze-thaws. | |
Full Length | |
STKR Kinase Family | |
Recombinant | |
Baculovirus |