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Invitrogen™ Exonuclease I
Useful in preparing PCR products for applications involving sequencing or labeling methods
Brand: Invitrogen™ 70073Z2500UN
490.69 SEK valid until 2024-03-29
Use promo code "21615" to get your promotional price.
Additional Details : Weight : 0.47500kg
Description
Exonuclease I is particularly useful in preparing the products of PCR for applications involving sequencing or labeling methods1. Typically, the excess primers and any other extraneous single-stranded DNA present in PCR products will interfere with subsequent enzymatic reactions involving DNA synthesis. The hydrolytic properties of Exonuclease I degrade all single-stranded DNA present in the PCR mixture allowing the product to be used more efficiently in other applications.
- Hydrolyzes single-stranded DNA in the 3′-5′ direction, releasing 5′-mononucleotides and leaving the terminal 5′-dinucleotide intact; hydrolysis is processive and cannot proceed if the 3′ terminus is phosphorylated
- Use to measure the endonucleolytic cleavage of covalently closed circular single-stranded DNA reacted with an endonuclease of interest2
- Also suitable for measuring DNA helicase activity3
- When combined with Shrimp Alkaline Phosphatase (Mfr. No. 78390) for dNTP dephosphorylation, the use of alternative purification methods, such as columns, gels or magnetic separations, are completely eliminated
Source: E. coli strain containing an overproducing clone of E. coli Exonuclease I
Properties:
- Molecular Weight: 55 kDa
- Heat Inactivation: 80° for 15 min.
- Degrades to terminal dinucleotides
- Degrades glycosylated DNA
- Optimum Temperature: 37°C
- Greater than 95% pure as determined by SDS-PAGE
- Tested for contaminating endonucleases, double-stranded exonucleases and ribonucleases
20mM Tris-HCI (pH 7.5), 5mM 2-mercaptoethanol, 0.5mM EDTA, 50% glycerol
Assay Conditions:
Reaction mixture (100μL) contains 67mM glycine buffer (pH 9.5), 10mM
2-mercaptoethanol, 6.7mM MgCl2, 0.5mM denatured DNA and enzyme
Concentration:
- Standard Conc.: 10 units/μL, Mfr. No. 70073
- High Conc.: 20 units/μL, Mfr. No. 72073
Elimination of residual single-stranded DNA containing a 3′ terminus; Measuring endonucleolytic cleavage of covalently closed circular ssDNA; Measuring DNA helicase activity
Specifications
10 U/μL | |
2500 U | |
Exonuclease I |