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Applied Biosystems™ AgPath-ID™ One-Step RT-PCR Reagents

AgPath-ID™ One-Step RT-PCR Reagents

Brand:  Applied Biosystems™ 4387424

Additional Details : Weight : 0.06800kg

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Product Code. 15574324

  • 8330.00 SEK / 500 reactions


In stock, ready to ship: 46
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Description

Description

AgPath-ID™ One-Step RT-PCR Reagents are designed for sensitive, robust amplification of RNA targets using a rapid single-tube TaqMan™ real-time reverse transcription PCR (RT-PCR) strategy.
  • Recommended for RNA pathogen amplification
  • Consistent amplification of RNA targets with high specificity and sensitivity
  • Optimized to work with your target-specific primers and probes
  • Contains ROX for quantitative fluorescent signal normalization

    AgPath-ID™ One-Step RT-PCR Reagents are configured for fast and simple reaction setup. The reactions are assembled in a single tube, minimizing sample handling errors and expediting set-up time. Once assembled, results are available in approximately one hour. The 25X RT-PCR Enzyme Mix included in the kit contains highly efficient ArrayScript™ Reverse Transcriptase, a mutant MMLV RT that produces high cDNA yields, and AmpliTaq Gold™ polymerase, the preferred hot-start DNA polymerase for specific target amplification. The 2X RT-PCR Buffer has been optimized for efficient, robust reverse transcription and PCR includes the passive reference dye, ROX™ Dye, for quantitative fluorescent signal normalization. A Detection Enhancer is also provided as an optional reagent for amplification of templates with a high GC content or persistent secondary structure.
  • Specifications

    Specifications

    Sufficient for 500 reactions of 25 µL, contains:
    • 7mL 2X RT-PCR Buffer
    • 550 µL 25X RT-PCR Enzyme Mix
    • 25 mL Nuclease-free Water.
    ROX (Pre-mixed)
    AgPath-ID™, Ambion™
    ArrayScript™
    Dry Ice
    1-Step qRT-PCR
    Standard
    7500 System
    TaqMan®
    Works on all AB platforms, but is optimized for the 7500.
    RNA
    Primer-Probe Detection
    AmpliTaq Gold DNA Polymerase
    Videos
    SDS
    Documents

    For Veterinary Use Only.