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TCR alpha/beta, PE, clone: WT31, eBioscience™

Mouse Monoclonal Antibody

858.00 SEK - 1904.00 SEK

Specifications

Antigen TCR alpha/beta
Clone WT31
Target Species Human
Conjugate PE
Isotype IgG1, kappa
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Products 2
Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
15537286 Affymetrix eBioscience
12-9955-41
25 Tests 858.00 SEK
25 tests View alternative products
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EU Stock 1
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15547286 Affymetrix eBioscience
12-9955-42
100 Tests 1904.00 SEK
100 tests View alternative products
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Estimated Shipment date
from Supplier 02-05-2019
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Description

Description

Description: This WT31 monoclonal antibody recognizes the human alpha beta T cell receptor (TCR) complexed with the CD3 epsilon chain. Composed of a 44-kDa alpha and a 37-kDa beta chain, TCR specificity is typically determined by V alpha, J alpha, V beta, D beta, and J beta gene rearrangement. The TCR is often associated with the CD3 complex, which is composed of at least five polypeptides. The TCR/CD3 complex recognizes and binds to major histocompatibility complex-bound antigen presented by antigen presenting cells such as B cells, dendritic cells, and macrophages. These events lead to T cell activation, resulting in cellular signaling and cytokine production.The WT31 monoclonal antibody has been reported to induce proliferation of resting T cells, as well as inhibit cytotoxic T cell activity. In addition, some antibodies against CD3 partially inhibit alpha beta TCR antibody staining using WT31; clone SK7 (anti-human CD3) is recommended for use in two-color experiments.Applications Reported: This WT31 antibody has been reported for use in flow cytometric analysis.Applications Tested: This WT31 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.06 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

TCR alpha/beta
Human
IgG1, kappa
Monoclonal
Liquid
Antibody
4° C, store in dark, DO NOT FREEZE!
WT31
PE
Flow Cytometry
Primary
Monoclonal
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
RUO

For Research Use Only.