Invitrogen™ alpha-Fetoprotein Monoclonal Antibody (AFP3), eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Description: This AFP3 monoclonal antibody reacts with human alpha-fetoprotein (AFP). This 70-kDa secretory protein is a member of the albumin gene family. Synthesized by the yolk sac and fetal liver during embryogenesis, AFP protein levels are highest in fetal serum. After birth, serum AFP levels decrease dramatically. In fact, AFP is nearly undetectable in normal adult serum. However, hepatocellular carcinoma and germ cell teratoblastoma, as well as liver regeneration, viral hepatitis, and cirrhosis, leads to elevated AFP serum levels in adults. As such, detection of this protein is frequently used as a diagnostic marker for these conditions. When performing western blotting or immunohistochemistry on paraffin section, we recommend the use of monoclonal antibody 1E8 (cat. 14-9499). Applications Reported: This AFP3 antibody has been reported for use in immunoblotting (WB) and immunocytochemistry. Applications Tested: This AFP3 antibody has been tested by western blot analysis of reduced HepG2 cell lysate, as well as by immunocytochemistry of HepG2 cells. For western blotting, this antibody can be used at 1-10 μg/mL. (Western blotting of non-reduced cell lysate yields a band at approximately 58 kDa.) For immunocytochemistry, AFP3 can be used at 1-5 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE.