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alpha GalCer:CD1d Complex Mouse anti-Mouse, PE, Clone: L363, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 12-2019-80

Additional Details : Weight : 0.25000kg

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Product Code. 15238719

  • 916.00 SEK / 25µg

Estimated Shipment: 28-07-2021

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Description

Description

Description: This L363 monoclonal antibody reacts with alpha-galactosylceramide bound to the mouse MHC class I-like protein CD1d. This complex is recognized by invariant natural killer T (iNKT) cells, a subset of T lymphocytes that expresses the invariant TCR alpha chain Valpha14Jalpha18. Activation of iNKT cells leads to production of Th1 and Th2-associated cytokines as well as dendritic cell maturation. The L363 antibody has been reported to neutralize iNKT cell activation by alpha-galactosylceramide:CD1d complexes. Applications Reported: This L363 antibody has been reported for use in flow cytometric analysis. Applications Tested: This L363 antibody has been tested by flow cytometric analysis of alpha-galactosylceramide-loaded mouse CD1d-transfected cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 μm post-manufacturing filtered.

This complex is recognized by invariant natural killer T (iNKT) cells, a subset of T lymphocytes that expresses the invariant TCR alpha chain Va14Ja18. Activation of iNKT cells leads to production of Th1 and Th2-associated cytokines as well as dendritic cell maturation.
Specifications

Specifications

alpha GalCer:CD1d complex
Monoclonal
0.2 mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P11609
Cd1d1
IgG2a, kappa
25 μg
4° C, store in dark, DO NOT FREEZE!
Primary
12479
Flow Cytometry
L363
PE
Cd1d1
Liquid
Mouse
Affinity chromatography
RUO
Antibody
Monoclonal
Mouse
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