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Invitrogen™ Decade™ Markers System

For making radiolabeled RNA markers

Brand:  Invitrogen™ AM7778

Additional Details : Weight : 0.00310kg

Product Code. 10299104

  • 2215.00 SEK / Each


EU Stock 4
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For Research Use Only. All usage must comply with product instructions.


Includes: Decade™marker RNA, Polynucleotide Kinase, Kinase Reaction Buffer, Cleavage Reagent, Gel Loading Buffer II, and Nuclease-free water

Description

Description

The Invitrogen™ Decade™ Marker System produces a set of radiolabeled RNA molecules of 150, 100, 90, 80, 70, 60, 50, 40, 30, 20, and 10 nucleotides. The molecular weight markers are ideally suited for experiments where RNA molecules less than 150 nucleotides in length are being studied. Sufficient reagents are provided for 10 marker preparation reactions. This marker set is particularly useful for research laboratories studying tRNA, snRNA, snoRNA, miRNA, siRNA and other small RNA molecules. The system includes all of the reagents necessary to produce the molecular weight markers except gamma-32P-ATP. The Decade™ Markers derive from a single, gel-purified transcript that is provided. The transcript is 5' end-labeled using the kinase and kinase buffer supplied with the kit. The kinase reaction is then diluted into a cleavage reagent that generates the molecular weight marker set in a five minute, room temperature reaction. Gel loading buffer from the kit is then added and the marker is ready for electrophoresis. The labeling, cleavage, and gel preparation steps can be completed in less than an hour. Compatible with denaturing polyacrylamide gels.

Acrylamide Gel Electrophoresis, DNA and RNA Purification and Analysis, Northern Blotting, Nuclease Protection Assays, Nucleic Acid Gel Electrophoresis and Blotting

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Shipping Condition: Dry Ice

Specifications

Specifications

100μg/ml
10 Reactions
10 to 150 bp
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For Research Use Only. Not for use in diagnostic procedures.