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Invitrogen™ mMESSAGE mMACHINE™ T7 Transcription Kit

in vitro synthesis of large amounts of capped RNA

Brand:  Invitrogen™ AM1344

7125.00 SEK valid until 2024-03-30
Use promo code "21741" to get your promotional price.



Additional Details : Weight : 0.06000kg


Product Code. 10438644

  • 7500.00 SEK / 25 reactions
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For Research Use Only. All usage must comply with product instructions.


Description

Description

mMESSAGE mMACHINE™ kits are designed for the in vitro synthesis of large amounts of capped RNA. Capped RNA mimics most eukaryotic mRNAs found in vivo, because it has a 7-methyl guanosine cap structure at the 5′ end.

  • RNA Yield: 20-30μg
  • Include cap analog [m7G(5′)ppp(5′)G] in ultra high-yield transcription reaction
  • Simplified reaction format in which all four ribonucleotides and cap analog are mixed in single solution
  • Cap analog: GTP ratio of solution is 4:1, which is optimal for maximizing both RNA yield and the proportion of capped transcripts
  • For routine synthesis of capped RNAs for oocyte microinjection, in vitro translation, transfection, and other applications
  • High yields are achieved by optimizing reaction conditions for RNA synthesis in presence of high nucleotide concentrations
  • RNA synthesized is protected from degradation by contaminating ribonucleases that present with RNase inhibitor - component of the Enzyme Mix

Specifications

Specifications

T7 Enzyme Mix, 10X Reaction Buffer 2XNTP⁄CAP Solution, GTP Solution, pTRI-Xef, TURBO DNase, Ammonium Acetate Stop Solution, Lithium Chloride Precipitation Solution, and Gel Loading Buffer II are all stored at –20°C. Nuclease-free Water may be stored at any temperature.
Kit
In Vitro Transcription, synthetic mRNA, mRNA, transfection, LNPs, GENEart, linearized DNA plasmid, delivery, PCR products
Ambion™, mMESSAGE mMACHINE™
25 Reactions
Dry Ice
PCR Products, Linearized DNA plasmid
Synthetic mRNA, mRNA
mRNA, Synthetic mRNA
25 Reactions
T7 In Vitro Transcription kit
T7
20-30 μg
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Safety and Handling

Safety and Handling

Product Identifier
  • mMESSAGE mMACHINE T7 Transcription Kit

Signal Word
  • Danger

Hazard Category
  • Acute toxicity Category 4
  • Carcinogenicity Category 2
  • Serious eye damage/eye irritation Category 2
  • Reproductive toxicity Category 1B
  • Skin corrosion/irritation Category 2
  • Specific target organ toxicity after repeated exposure Category 2

Hazard Statement
  • H302-Harmful if swallowed.
  • H315-Causes skin irritation.
  • H319-Causes serious eye irritation.
  • H351-Suspected of causing cancer.
  • H360-May damage fertility or the unborn child.
  • H373-May cause damage to organs.

Precautionary Statement
  • P201-Obtain special instructions before use.
  • P202-Do not handle until all safety precautions have been read and understood.
  • P260-Do not breathe dust/fume/gas/mist/vapours/spray.
  • P264-Wash hands thoroughly after handling.
  • P270-Do not eat, drink or smoke when using this product.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
  • P302+P352-IF ON SKIN: Wash with plenty of water/soap.
  • P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
  • P308+P313-IF exposed or concerned: Get medical advice/attention.
  • P314-Get medical advice/attention if you feel unwell.
  • P330-Rinse mouth.
  • P332+P313-If skin irritation occurs: Get medical advice/attention.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information
  • MIXTURE LIST-Contains : lithium chloride, formamide

SDS
Documents

Documents

Certificates
Special Offers

Special Offers

For Research Use Only. Not for use in diagnostic procedures.