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Invitrogen™ RNAlater™ Stabilization Solution

Immediate RNase inactivation and RNA stabilization within tissues or cells

1390.00 SEK - 5080.00 SEK

Specifications

Product Type RNAlater™ Stabilization Solution
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Products 7
Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
10620036 Invitrogen™
AM7022
50 x 1.5mL 3040.00 SEK
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10391085 Invitrogen™
AM7024
1 x 250mL 3295.00 SEK
250mL
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10381085 Invitrogen™
AM7023M
20 x 5mL 3450.00 SEK
100mL
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10066334 Invitrogen™
AM7022M
50 x 1.5mL 3155.00 SEK
75mL
Estimated Shipment date
from Supplier 06-02-2019
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10564445 Invitrogen™
AM7020
100mL 1390.00 SEK
100mL
EU Stock 62
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10594445 Invitrogen™
AM7023
20 x 5mL 3230.00 SEK
100mL
EU Stock 25
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10427114 Invitrogen™
AM7021
500mL 5080.00 SEK
500mL
EU Stock 106
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Description

Description

Independent research, indicates that the use of RNAlater RNA Stabilization Solution for tissue storage does not affect the outcome of subsequent RNA expression analysis experiments compared to other processing methods. RNAlater™ RNA Stabilization Solution stabilizes and protects cellular RNA in intact, unfrozen tissue samples, eliminating the need to immediately process tissue samples or to freeze samples in liquid nitrogen for later processing. Tissue pieces can be harvested and submerged in RNAlater RNA Stabilization Solution for storage without jeopardizing the quality or quantity of RNA obtained after subsequent RNA isolation.

  • Effectiveness: stabilize RNA for 1 day at 37°C, 1 week at 25°C, 1 month at 4°C, or indefinitely at -20°C
  • Simplicity: a single reagent that immediately inactivates RNases and stabilizes RNA within tissues or cells
  • Convenience: no need to freeze samples in liquid nitrogen or rush samples back to the lab freezer
  • Mobility: perfect for tissue collection in the field
  • Versatility: compatible with many RNA isolation procedures, including most RNA isolation kits
RNAlater RNA Stabilization Solution procedure
The dissected tissue (less than 0.5cm in any one dimension) is simply submerged in approximately 5 volumes of RNAlater solution at room temperature. The solution permeates the cells, stabilizing the RNA. The sample can then be stored indefinitely at -20°C (the tissue does not freeze), at 4°C for up to a month, or at 25°C for up to a week. For RNA isolation, the tissue is simply removed from RNAlater solution and treated as though it had just been harvested. Most tissues can be transferred directly to a lysis buffer and homogenized. Samples treated with RNAlater solution and then frozen can be ground with mortar and pestle or thawed and processed like fresh tissue without concern for cell rupture and release of RNases since the RNases have already been inactivated. Cells can be spun out and then added to lysis buffer, or in some cases, RNAlater solution can be added along with the cells directly to the lysis buffer.
Compatible with a variety of procedures RNAlater RNA Stabilization Solution is compatible with one-step RNA isolation methods, such as TRIzol Reagent; with glass binding methods such as Qiagen's RNeasy™ or the Ambion RNAqueous kit; with acid phenol extraction methods such as the Ambion ToTALLY RNA™ kit; and with methods that use oligo(dT) selection of mRNA, such as the Ambion Poly(A)Purist™ kit.

Applications

  • RNAlater RNA Stabilization Solution has been tested on a variety of mammalian tissues, plants, E. coli, Xenopus, fish, and Drosophila.
  • Protecting RNA integrity in tissues rich in RNases
  • Collecting samples from different time points without having to process the samples from each time point immediately
  • Archiving tissues for future microdissection
  • Submerging animal cavities or organs to stabilize RNA during lengthy dissection procedures
  • Collecting samples at locations (e.g., hospitals, field sites, the space shuttle) where immediate RNA isolation is not possible
  • Shipping samples on wet ice or even at room temperature if shipped overnight

DNA and RNA Purification and Analysis, General RNA Purification Reagents and Accessories, RNA Extraction, Total RNA Isolation, Total RNA from Animal Cells and Tissues, mRNA Isolation

Order Info

Shipping Condition: Room temperature

Specifications

Specifications

RNAlater™ Stabilization Solution

For Research Use Only. Not for use in diagnostic procedures.