Invitrogen™ RNAqueous™-Midi Total RNA Isolation Kit
RNA isolation for midrange amounts of starting material
Brand: Invitrogen™ AM1911
Additional Details : Weight : 1.08000kg
The Ambion RNAqueous-Midi Kit is for phenol-free total RNA isolation from 0.1g to 0.5g of tissue or 107 to 108 cells using a guanidinium-based lysis/;denaturant and glass fiber filter separation technology. The RNAqueous-Midi Kit provides enough reagents for 15 RNA purifications. The Ambion RNAqueous-Midi Kit uses a powerful chaotropic solution for cell lysis, followed by RNA binding to glass fiber housed in a luer-lock syringe filter. Washing and elution solutions are simply pushed through the filter using a standard syringe (not included). Vacuum filtration is not required; however, the filter units are compatible with standard vacuum manifolds for processing several samples at once.
- Convenient syringe-based filtration
- Compatible with vacuum manifolds when processing multiple samples
Not high-throughput compatible (Manual)
DNA and RNA Purification and Analysis, RNA Extraction, Total RNA Isolation, Total RNA from Animal Cells and Tissues
|Formaldehyde Load Dye, Lysis/Binding Solution, Water for 64% Ethanol, Lithium, Chloride Solution, Wash Solution No. 1 and Wash Solution 2/3 Concentrate, Syringe Filter Units, Collection Tubes and Elution Solution|
|Not High-throughput Compatible (Manual)|
|Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Nuclease Protection Assays, Northern Blotting, cDNA Library Construction|
|Syringe Filter (Glass Fiber)|
Formaldehyde Load Dye should bestored at -20°C; Lysis/Binding Solution, Water for 64% Ethanol, LithiumChloride Solution, Wash Solution #1 and Wash Solution 2/3 Concentrate should bestored at 4°C; Syringe Filter Units, Collection Tubes and Elution Solution canbe stored at room temperature. All reagents are guaranteed stable for 6 months whenproperly stored.