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Thermo Scientific™ AminoLink™ Coupling Resin Product Code.: 11826774
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Thermo Scientific™ AminoLink™ Coupling Resin

Product Code. 11826774 Shop All Thermo Scientific Products
10 mL, 10mL
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Quantity:
10 mL
50 mL
Unit Size:
10mL
50mL
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Product Code. 11826774

Brand: Thermo Scientific™ 20381

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This item is not returnable. View return policy

Description

Covalently immobilize proteins and antibodies by lysine residues or other primary amines (-NH2) via reductive amination with these aldehyde-activated agarose beads.

Thermo Scientific™ AminoLink Coupling Resin is crosslinked 4% beaded agarose that has been activated with aldehyde groups to enable covalent immobilization of antibodies and other proteins through primary amines.

AminoLink Coupling Resin is crosslinked 4% beaded agarose that has been activated with aldehyde groups. Proteins and other molecules with primary amines can be covalently attached (immobilized) to the resin to make chromatography columns for use in affinity purification. The aldehyde groups form stable secondary amine bonds with primary amines such as exist in the side chain of lysine (K) residues, which are generally abundant and readily accessible in proteins. Once a protein is immobilized, the prepared affinity resin can be used for a variety of batch and column affinity purification methods involving binding interactions with the immobilized protein. The resin and linkage are stable in most binding and elution conditions typically used in affinity chromatography, enabling prepared resin to be used for multiple rounds of affinity purification procedures.

Highlights:

  • AminoLink Coupling Resin – aldehyde-activated crosslinked 4% beaded agarose

  • Ideal for antibodies and other proteins – immobilize molecules via primary amines (-NH2)

  • Flexible coupling conditions – efficient (>85%) coupling over a wide range of pH (4 to 10) and buffer conditions (PBS or other non-amine buffer with or without organic solvent

  • Stable, permanent immobilization – coupling reaction results in stable, leak-resistant secondary amine bond between resin and ligand

  • Better than immobilization to CNBr-activated agarose – bond is more stable and uncharged, resulting in less nonspecific binding in affinity purification procedures

  • Versatile and reusable – prepared affinity resin is adaptable to column and batch affinity techniques and the resin is reusable for typical applications based on protein binding interactions

Includes:

Kit contains pre-packed AminoLink Columns, coupling buffer, quenching buffer, wash solution, sodium cyanoborohydride solution, and column accessories

TRUSTED_SUSTAINABILITY

Specifications

Content And Storage Store at 4°C.
Product Line AminoLink™
Quantity 10 mL
Target Antibody, Proteins through Primary Amine
Type Resin
Stationary Phase Aldehyde
Format Bottle
Column Type Affinity, Agarose Resin

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5 results found
Lot Number Certificate Type Date Product Code
Lot NumberZJ399290 Certificate TypeCertificate of Analysis Date15/10/2024 Product Code20381
Lot NumberZD383510 Certificate TypeCertificate of Analysis Date30/04/2024 Product Code20381
Lot NumberYG372919 Certificate TypeCertificate of Analysis Date10/08/2023 Product Code20381
Lot NumberYA360933 Certificate TypeCertificate of Analysis Date11/01/2023 Product Code20381
Lot NumberXD344676 Certificate TypeCertificate of Analysis Date01/06/2022 Product Code20381

For Research Use Only. Not for use in diagnostic procedures.

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