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AMPK A2/B1/G1 Recombinant Human Protein

AMPK A2/B1/G1 Recombinant Human Protein

Brand:  Invitrogen PR8694C

Additional Details : Weight : 0.01000kg

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Product Code. 15448683

  • 21350.00 SEK / 100µg


Estimated Shipment date
from Supplier 04-01-2018
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Disclaimers:

For Research Use Only. Not for use in diagnostic procedures.


Description

Description

AMP-activated protein kinase (AMPK) is a heterotrimeric complex that acts as a sensor of cellular energy levels. The signaling cascades initiated by activating AMPK are critical to regulating metabolic events in the liver, skeletal muscle, heart, adipose tissue, and pancreas. An impairment in fuel metabolism that occurs in obesity is a factor leading to Type 2 diabetes. The insulin resistance associated with type 2 diabetes is most profound at the level of skeletal muscle, the primary site of glucose and fatty acid utilization. Activation of AMPK is of interest for the treatment of type 2 diabetes.
Specifications

Specifications

NP_0062243.2, NP_006244.2, NP_002724.1
Protein assays and analysis
Frozen
AMPK A2/B1/G1 Recombinant Human Protein
100μg
For research use only. Not for use in diagnostic procedures
AMPK A2/B1/G1
AMPK (A2/B1/G1) was pre-diluted in enzyme dilution buffer and assayed in 25mM HEPES (pH 7.5), 10mM MgCl2, 0.5mM EGTA, 0.5mM Na3VO4, 5mM β-glycerophosphate, 2.5mM DTT, 0.01% Triton™ X-100, 500μM ATP, 200μM SAMS peptide substrate (HMRSAMSGLHLVKRR), 150μM AMP and trace [32P]-γ-ATP for 10 minutes at 30°C
Baculovirus
AMPK A2/B1/G1 Recombinant Human Protein
Human
0.35mg/mL
Frozen
190.9kDa
90% by SDS-PAGE
–80°C
1 Each
AMPK A2/B1/G1 Recombinant Human Protein
Kinase assay
CAMKL
6,260nmoles of phosphate transferred to SAMS peptide substrate (HMRSAMSGLHLVKRR) per minute per mg of total protein at 30°C. Activity determined at a final protein concentration of 0.21μg/mL. Activity increased 3.2 fold by using 150μM AMP
GST Tag, His Tag
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