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STEMCELL Technologies Annexin V, FITC

Produktkod. 17157561
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Produktkod. 17157561 Leverantör STEMCELL Technologies Leverantörsnummer 1000333

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Beskrivning

Assay buffer for labeling with Annexin V

  • Annexin V is a member of the annexin family of proteins that bind to membrane phospholipids in the presence of calcium
  • This dye has high affinity for phosphatidylserine (PS) that is present in the inner leaflet of the plasma membrane
  • During early-stage cell apoptosis, PS is translocated from the inner to the outer leaflet of the plasma membrane, exposing it to the external environment
  • Annexin V, a characteristic marker for early cell apoptosis, detects the translocation of PS to the external environment
  • Annexin V is used along with viability dyes such as 7-AAD (Catalog #75001) or Propidium Iodide (Catalog #75002)
  • The process of PS translocation occurs prior to the loss of membrane integrity
  • Therefore, as cells progress through apoptosis and towards necrosis, the cell membrane is compromised and consequently, viability dyes pass into the cell
  • Thus, cells undergoing early apoptosis stain positive for Annexin V and negative for viability dyes, while apoptotic death or necrosis is characterized by positive staining for both Annexin V and the viability dye

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Kvantitet 100 tester
För användning med (applikation) Flow Cytometry
Konjugera FITC
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