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CD28, Functional Grade, clone: CD28.6, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 16-0288-85

Additional Details : Weight : 0.01000kg

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Product Code. 15268417

  • 2835.00 SEK / 500µg


EU Stock 11
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Description

Description

Description: The CD28.6 monoclonal antibody reacts with the human CD28 molecule, a 44kDa homodimer expressed by thymocytes, mature T cells and plasma cells. CD28 is a ligand for CD80 (B7-1) and CD86 (B7-2) and is a potent costimulator of T cells. Signaling through CD28 augments IL-2 and IL-2 receptor expression as well as cytotoxicity of CD3-activated T cells. The CD28.6 antibody has been reported for use in immunoblotting (WB) and neutralization studies and costimulation in the production of IL-2.Applications Reported: The CD28.6 antibody has been reported for use in flow cytometric analysis, and immunoblotting (WB). The recommended titer for WB is (∽1 μ/mL). CD28.6 has also been reported in in vitro functional assays, both blocking and co-stimulatory activities have been reported for this clone (please see references below).Applications Tested: TheCD28.6 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay.Storage and handling: Use in a sterile environment.Filtration: 0.2μm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.
Specifications

Specifications

CD28
Monoclonal
1mg/mL
PBS with no preservative; pH 7.2
P10747
MGC138290, Tp44, CD28 antigen, T-cell-specific surface glycoprotein CD28
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow CytometryþFunctional AssayþWestern Blot
CD28.6
Functional Grade
CD28
Purified
CD28
IgG2a, kappa
500μg
Store at 2-8°C.
Primary
940
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