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CD45 Rat anti-Mouse, Brilliant Violet 510, Clone: 30-F11, BD
Rat Monoclonal Antibody
Brand: BD Biosciences 563891
Beskrivning
The 30-F11 clone has been reported to react with all isoforms and both alloantigens of CD45, which is found on hematopoietic stem cells and all cells of hematopoietic origin, except erythrocytes. CD45 is a transmembrane glycoprotein which is expressed at high levels on the cell-surface, and its presence distinguishes leukocytes from non-hematopoietic cells. CD45 is a member of the Protein Tyrosine Phosphatase (PTP) family, where the intracellular carboxy-terminal region contains two PTP catalytic domains, and the extracellular region is highly variable due to alternative splicing of exons 4, 5, and 6 (designated as A, B, and C, respectively). CD45 isoforms play complex roles in T-cell and B-cell antigen receptor signal transduction and the CD45 isoforms detected in the mouse are cell type-, maturation-, and activation state-specific.
The antibody was conjugated to BD Horizon™ BV510 which is part of the BD Horizon™ Brilliant Violet™ family of dyes. With an Ex Max of 405-nm and Em Max at 510-nm, BD Horizon™ BV510 can be excited by the violet laser and detected in the BD Horizon™ V500 (525/50-nm) filter set. BD Horizon™ BV510 conjugates are useful for the detection of dim markers off the violet laser.
Specifikationer
| CD45 | |
| Monoclonal | |
| 0.2mg/mL | |
| Ptprc; CD45R; LCA; Leukocyte common antigen; T200; B220; Ly-5; Lyt-4 | |
| Rat | |
| Affinity Purified | |
| RUO | |
| Mouse | |
| IgG2b κ |
| Flow Cytometry | |
| 30-F11 | |
| Brilliant Violet 510 | |
| Aqueous buffered solution containing BSA and ≤0.09% sodium azide. | |
| Mouse Thymus / Spleen | |
| 50 μg | |
| Primary | |
| Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. |
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