anti-GFP Tag FITC, Polyclonal, Invitrogen™

Beskrivning

GFP Tag Polyclonal antibody PA1-46326 from Thermo Scientific™ specifically detects GFP Tag in Tag samples. It is validated for Flow Cytometry, Immunocytochemistry, Immunofluorescence and Western Blot.

Green fluorescence protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology.
Host Species: Rabbit
Cross Reactivity: Tag
Isotype: IgG
Immunogen: Green fluorescent protein (GFP) fusion protein corresponding to the full length sequence (246 aa) from the jellyfish Aequorea victoria.
Formulation: 50μg Purified Antibody (1mg/ml) in 0.02M potassium phosphate, with 0.15M NaCl, 10mg/ml BSA and 0.01% sodium azide; pH7.2
Synonyms: Green Fluorescence Protein
This antibody shows minimal cross reactivity to human, mouse, and rat serum proteins.