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Armenian Hamster IgG, Isotype Control, Invitrogen™

Armenian Hamster Isotype Control

Brand:  Invitrogen PA533219

Additional Details : Weight : 0.01000kg

Product Code. 15354411

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Description

Description

The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.

PA5-33219 is suitable for use as a negative control for the measurement of non-specific binding of Armenian hamster monoclonal antibodies, of isotype IgG, in human, mouse or rat tissues.For FACS analysis, use 10ul of the suggested working dilution to label 1x10^6 cells in 100ul.
Specifications

Specifications

Armenian Hamster IgG
0.5mg/mL
IgG
Trinitrophenol - KLH
250 μg
Isotype Control (Primary)
Store at 4°C or -20°C if preferred
IgG
Control, Flow Cytometry
Unconjugated
Armenian Hamster
Ion-exchange chromatography
RUO
Not Applicable
Liquid
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