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S6 Rabbit anti-Human, Polyclonal, Invitrogen™

Rabbit Polyclonal Antibody

Brand:  Invitrogen PA532578

Additional Details : Weight : 0.01000kg

Product Code. 13407097

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Description

Description

Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is breast carcinoma.

40S ribosomal protein S6 (also known as RPS6) is a ∽31 kDa substrate of p70 S6 kinase (p70S6K) and a major component of translational machinery involved in protein synthesis, cell growth, proliferation, and metabolism. RPS6 undergoes phosphorylation on multiple serines in the carboxyl terminal region in the order 236-->235-->240-->244-->247, due to the positions of these amino acid residues on the alpha-helix. Hyperphosphorylation of RPS6 stimulates protein synthesis that mediates progression through the cell cycle.
Specifications

Specifications

S6
Polyclonal
RPS6
S6, 40S ribosomal protein S6, phosphoprotein NP33
Rabbit
Antigen affinity chromatography
RUO
6194
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Liquid
Immunohistochemistry (Paraffin), Western Blot
Unconjugated
P62753
RPS6
Synthetic peptide derived from the region of human S6 ribosomal that contains unphosphorylated serines 235 and 236.
500 μL
Primary
Human
Antibody
IgG
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