BCL6 Rat anti-Mouse, PerCP-eFluor™ 710, Clone: BCL-DWN, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 46-5453-80
Additional Details : Weight : 0.09500kg
DescriptionIt is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser. Filtration: 0.2 μm post-manufacturing filtered. Bcl-6 proto-oncogene product (a Kruppel-type zinc-finger protein) is mainly expressed in normal germinal center B cells and related lymphomas. Bcl-6 is involved in chromosome rearrangements at 3q27 in non-Hodgkin′s lymphomas and Bcl-6 rearrangements have been detected in 33%-45% of diffuse large B cell lymphomas. Bcl-6 has been detected immunohistochemically in follicular lymphomas, diffuse large B cell lymphomas, Burkitt′s lymphomas and in nodular, lymphocyte predomit Hodgkin′s disease.
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|B-cell lymphoma 6 protein; BCL-6; Zinc finger protein 51; LAZ-3 protein; BCL-5; Zinc finger and BTB domain-containing protein 27; ZNF51; ZBTB27; LAZ3; BCL5; BCL6|
|4° C, store in dark, DO NOT FREEZE!|
For Research Use Only.