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BD Difco™ Yeast Nitrogen Base without Amino Acids

Used for classifying yeasts based on amino acid and carbohydrate requirements. BD™ Difco™ Yeast Nitrogen Base without Amino Acids is a dehydrated culture medium used in many molecular genetics applications. Prepared according to the formulas of Wickerham.

Brand:  BD 291940

Additional Details : Weight : 0.13500kg

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Product Code. 11753573

Quantity Price
1 1793.00 SEK / 100g
10 1574.79 SEK / 100g
In stock, ready to ship: 2
In stock, ready to ship within 7 days: 42
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Yeast Nitrogen Base without Amino Acids contains all essential vitamins and inorganic salts necessary for the cultivation of yeasts except histidine, methionine, tryptophan and a source of carbohydrate.

  • Prepared according to Guenter’s modification of Wickerham’s Yeast Nitrogen Base formulation
  • Good cultural response with Kloeckera apiculata 9774 and Saccharomyces cerevisiae 9080
  • pH (0.67% solution, 25°C): 5.4 ± 0.2

Yeasts are unicellular, eukaryotic, budding cells that are generally round-to-oval or elongate in shape. They multiply principally by the production of blastoconidia (buds). Yeast colonies are moist and creamy or glabrous to membranous in texture. Yeasts are considered opportunistic pathogens.



White to off-white
For classifying Yeasts based on Nitrogen assimilation
Yeast Nitrogen Base without Amino Acids
Nitrogen Source:  Ammonium sulfate 5g/L
Vitamins: Biotin 2μg/L, Calcium pantothenate 400μg/L, Folic acid 2μg/L, Inositol 2000μg/L, Niacin 400μg/L, p-Aminobenzoic Acid 200μg/L, Pyridoxine hydrochloride 400μg/L, Riboflavin 200μg/L, Thiamine hydrochloride 400μg/L
Compounds Supplying Trace Elements: Boric acid 500.0μg/L, Copper sulfate 40.0μg/L, Potassium iodide 100.0μg/L, Ferric chloride 200.0μg/L, Manganese sulfate 400.0μg/L, Sodium molybdate 200.0μg/L, Zinc sulfate 400.0μg/L
Salts: Monopotassium phosphate 1.0g/L, Magnesium sulfate 0.5g/L, Sodium chloride 0.1g/L, Calcium chloride 0.1g/L
Yeast Nitrogen base without Amino Acids
1825 Days
Dehydrated Powder
ISO, 9000