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Phosflow Lyse/Fix Buffer 5X, BD

For the lysing and fixing of whole blood for use with intracellular flow cytometry

Brand:  BD Biosciences 558049

Additional Details : CAS Number : 50-00-0 Weight : 0.25000kg Transport : UN number : 2209 Chem class : 8 Pack group : III

Product Code. 15839178

  • 6955.00 SEK / Each
Estimated Shipment: 01-08-2024
to see stock.



  • Can be used for lysing red blood cells and for fixing leukocytes in one step, allowing investigators to rapidly inactivate kinases, phosphatases and/or proteases, so that the in vivo phosphorylation state of the cell can be examined
  • Has been reported to preserve the light scattering properties of the cell
  • May be used on human and mouse whole blood
  • Whole blood lysis has been shown to be as effective as density gradient centrifugation in the preparation of PBMCs for lymphocyte subset analysis
  • In many cases, phospho-specific antibodies may be used simultaneously with the staining of cell surface markers (e.g. CD markers); however, not all cell surface markers, however, are compatible with the BD Phosflow Lyse/Fix Buffer



Room Temperature
Lyse/Fix Buffer
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Safety and Handling

Safety and Handling

Product Identifier
  • BD Phosflow Lyse/Fix Buffer

Signal Word
  • Danger

Hazard Category
  • Acute toxicity Category 3
  • Acute toxicity Category 4
  • Carcinogenicity Category 1B
  • Serious eye damage/eye irritation Category 2
  • Germ cell mutagenicity Category 2
  • Skin corrosion/irritation Category 2
  • Skin sensitiser Category 1
  • Specific target organ toxicity after repeated exposure Category 2
  • Specific target organ toxicity Category 2

Hazard Statement
  • H241-Heating may cause a fire or explosion.
  • H302-Harmful if swallowed.
  • H311-Toxic in contact with skin.
  • H315-Causes skin irritation.
  • H317-May cause an allergic skin reaction.
  • H319-Causes serious eye irritation.
  • H331-Toxic if inhaled.
  • H335-May cause respiratory irritation.
  • H350-May cause cancer.
  • H371-May cause damage to organs.
  • H373-May cause damage to organs.

Precautionary Statement
  • P201-Obtain special instructions before use.
  • P260-Do not breathe dust/fume/gas/mist/vapours/spray.
  • P264-Wash thoroughly after handling.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P308+P313-IF exposed or concerned: Get medical advice/attention.
  • P403+P233-Store in a well-ventilated place. Keep container tightly closed.

Supplemental information
  • MIXTURE LIST-Contains : formaldehyde, diethylene glycol, methanol



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