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beta Catenin, clone: 15B8, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-2567-80

Additional Details : Weight : 0.01000kg

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Product Code. 15247327

  • 672.00 SEK / 25µg


EU Stock 2
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Description

Description

Description: The 15B8 monoclonal antibody reacts with human and mouse beta-catenin, one member of a family of catenins, which are intracellular proteins that interact with cadherins to mediate cellular adhesion. More specifically, beta-catenin binds to the cytoplasmic tail of E-cadherin. In addition, this molecule is a component of the canonical Wnt signaling pathway. In the absence of Wnt binding its receptor, beta-catenin is phosphorylated and resides in the cytoplasm where it is eventually targeted for degradation by ubiquitination. Upon Wnt binding, beta-catenin becomes dephosphorylated, translocates to the nucleus, and modulates gene expression in partnership with the transcription factors T cell factor (TCF) and lymphocyte enhancer binding factor (LEF). Expression of beta-catenin is found in a wide variety of non-immune and immune tissues, including thymocytes and T and B lymphocytes. The Wnt and beta-catenin signaling pathway has been demonstrated to play a crucial role in the development of T, B, and hematopoietic stem cells.Applications Reported: This 15B8 antibody has been reported for use in intracellular staining followed by flow cytometric analysis, immunoprecipitation, western blotting, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections. (Fluorochrome conjugated 15B8 is recommended for use in intracellular flow cytometry.).Applications Tested: This 15B8 antibody has been tested by western blot analysis on reduced cell lysates prepared from the Jurkat cell line. This antibody can be used at 1-5 μg/mL. This antibody has been tested by immunohistchemistry of formalin-fixed paraffin embedded human tissue using low or high pH antigen retrieval and can be used at less than or equal to 5 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

beta Catenin
Monoclonal
0.5mg/mL
PBS with 0.09% sodium azide; pH 7.2
P35222, Q02248
Cadherin associated protein, Catenin beta 1, CATNB, CTNNB
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
HumanþMurine
Flow CytometryþImmunohistochemistry (Paraffin)þImmunoprecipitationþWestern Blot
15B8
Unconjugated
CTNNB1
Liquid
CTNNB1
IgG1, kappa, kappa
25μg
Store at 2-8°C.
Primary
12387, 1499
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