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Invitrogen™ Beta-Lactamase (TEM-1) Recombinant Protein
Beskrivning
TEM-1 beta-lactamase is the most widespread enzyme to confer beta-lactam antibiotic resistance to gram-negative bacteria. It is a monomeric protein that requires no metals or cofactors for activity. TEM-1 beta-lactamase efficiently hydrolyzes most penicillin and cephalosporin substrates, including nitrocefin and fluorescent derivates. This protein is intended to be used as a positive control for enzyme-dependent substrate hydrolysis and for use on immunoblots. Other uses include kinetic studies with novel substrates or identification of inhibitors of beta-lactamase as part of an antimicrobial program. 1165 micromoles of penicillin G hydrolyzed per minute per milligram of total protein.
Specifikationer
Specifikationer
| Tillträdesnummer | Q9HCU0 |
| Koncentration | See Label |
| För användning med (applikation) | Kinase Assay |
| Formulering | 200mM potassium phosphate with 50% glycerol and no preservative; pH 7 |
| Gen-ID (Entrez) | 57124 |
| Molekylvikt (g/mol) | 38 kDa |
| Namn | Beta-Lactamase (TEM-1) |
| pH-intervall | 7 |
| Reningsmetod | Purified |
| Kvantitet | 10 μg |
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