Learn More
Applied Biosystems™ BigDye™ Direct Cycle Sequencing Kit
Shop All Applied Biosystems ProductsDescription
Includes
This kit contains BigDye™ Direct PCR Master Mix, Sequencing Master Mix, M13 Fwd Primer, M13 Rev Primer, and Control DNA CEPH 1347-02, This kit contains sufficient reagents for 24 reactions
- A streamlined, simple procedure
- Performance of all sequencing steps in a single reaction tube
- Fast migration with excellent resolution starting at the 1st base after the gene-specific primer sequence
- Substantial time savings versus previous workflows
Order Info
1 Kit

Specifications
Specifications
Content And Storage | This kit contains BigDye™ Direct PCR Master Mix, BigDye™ Direct Sequencing Master Mix, BigDye™ Direct M13 Fwd Primer, BigDye™ Direct M13 Rev Primer, and Control DNA CEPH 1347-02. Store all components at -15 to -25°C and protect from light. PCR Master Mix should be stored at 4°C after first use. This kit contains sufficient reagents for 100 reactions. |
Format | 8-well Strip, 96-well Plate |
For Use With (Equipment) | 3130xl Genetic Analyzer, 3730xl DNA Analyzer, GeneAmp 9700, SeqStudio Genetic Analyzer, Veriti Thermal Cycler, 3500 Genetic Analyzer, 3500xL Genetic Analyzer, 3730 DNA Analyzer |
For Use With (Application) | Comparative Sequencing (Germline Mutations - 30:70 Heterozygotes), Comparative Sequencing (Germline Mutations - 50:50), GC-Rich Sequencing (>65%), GT-Rich or Difficult Template Sequencing, Standard Sequencing, Gap Closure, Homopolymer A or T, AT-Rich Sequencing (>65%), Comparative Sequencing (Germline Mutations -10:90 Heterozygotes) |
Includes | BigDye™ Direct Cycle Sequencing Kit, 100 rxns, 8-well Strips, 96-well Plate, Fluorescent Dye Terminator Sequencing Technique, 3, Use with 130xl Genetic Analyzer, 3500 Genetic Analyzer, 3500xL Genetic Analyzer, 3730 DNA Analyzer, 3730xl DNA Analyzer, GeneAmp™ 9700, Veriti™ Thermal Cycler |
No. of Reactions | 100 Reactions |
Product Line | BigDye™ |
Product Type | Direct Cycle Sequencing Kit |
Quantity | 100 reactions |
Shipping Condition | Dry Ice |
Show More |
Documents
Product Information
Manuals
Specification Sheets
Scientific Resources
Brochures
Reference Materials
Certificates
Certificates
A lot number is required to show results for certificates. To find your lot number on previous orders use our order status area.
Lot Number | Certificate Type | Date | Product Code |
---|---|---|---|
2843417 | Certificate of Analysis | 31/01/2025 | 4458687 |
2829650 | Certificate of Analysis | 29/01/2025 | 4458687 |
2816385 | Certificate of Analysis | 29/01/2025 | 4458687 |
2829639 | Certificate of Analysis | 29/01/2025 | 4458687 |
2792553 | Certificate of Analysis | 29/01/2025 | 4458687 |
Promotions
-
Multiple Manufacturers
Genomics Powerhouse
Power your pipeline from PCR to sequencing.Promo Code: 24117Expires: 2025-08-24
New Offer Save Up to 92%
For Research Use Only. Not for use in diagnostic procedures.
Your input is important to us. Please complete this form to provide feedback related to the content on this product.