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Applied Biosystems™ BigDye™ Direct Cycle Sequencing Kit
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No. of Reactions:
100 Reactions
1000 Reactions
24 Reactions
Unit Size:
100 reactions
1000 reactions
24 reactions
Description
Includes
This kit contains BigDye™ Direct PCR Master Mix, Sequencing Master Mix, M13 Fwd Primer, M13 Rev Primer, and Control DNA CEPH 1347-02, This kit contains sufficient reagents for 24 reactions
- A streamlined, simple procedure
- Performance of all sequencing steps in a single reaction tube
- Fast migration with excellent resolution starting at the 1st base after the gene-specific primer sequence
- Substantial time savings versus previous workflows
Order Info
1 Kit

Specifications
Specifications
Content And Storage | This kit contains BigDye™ Direct PCR Master Mix, Sequencing Master Mix, M13 Fwd Primer, M13 Rev Primer, and Control DNA CEPH 1347-02. Store all components at -15 to -25°C and protect from light. PCR Master Mix should be stored at 4°C after first use. This kit contains sufficient reagents for 1000 reactions. |
Format | 8-well Strip, 96-well Plate |
For Use With (Equipment) | 3130xl Genetic Analyzer, 3730xl DNA Analyzer, GeneAmp 9700, SeqStudio Genetic Analyzer, Veriti Thermal Cycler, 3130 Genetic Analyzer, 3730 DNA Analyzer, 3500xL Genetic Analyzer, 3500 Genetic Analyzer |
For Use With (Application) | Comparative Sequencing (Germline Mutations - 30:70 Heterozygotes), Comparative Sequencing (Germline Mutations - 50:50), GC-Rich Sequencing (>65%), GT-Rich or Difficult Template Sequencing, Standard Sequencing, Gap Closure, Homopolymer A or T, AT-Rich Sequencing (>65%), Comparative Sequencing (Germline Mutations -10:90 Heterozygotes) |
Includes | BigDye™ Direct Cycle Sequencing Kit, 1000 rxns, 8-well Strips, 96-well Plate, Fluorescent Dye Terminator Sequencing Technique, 3, Use with 130xl Genetic Analyzer, 3500 Genetic Analyzer, 3500xL Genetic Analyzer, 3730 DNA Analyzer, 3730xl DNA Analyzer, GeneAmp™ 9700, Veriti™ Thermal Cycler |
No. of Reactions | 1000 Reactions |
Product Line | BigDye™ |
Product Type | Direct Cycle Sequencing Kit |
Quantity | 1,000 reactions |
Shipping Condition | Dry Ice |
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Documents
Product Information
Manuals
Protocol: BigDye® Direct Cycle Sequencing Kit (English )
Version: 08 Feb 2011
File size: 1 MB
Product Insert: BigDye Direct Cycle Sequencing Kit
Version: C
File size: 565.1 kB
User Bulletin: Generating high-quality data using the BigDye Direct Cycle Sequencing Kit
Version: MAN0014436 Rev.A (15Jan2016)
File size: 417.3 kB
Quick Reference Card: BigDye® Direct Cycle Sequencing Kit (English )
Version: 08 Feb 2011
File size: 315.1 kB
Troubleshooting guide: FAQ: BigDye Direct Cycle Sequencing Kit PDF (English )
Version: 30 Mar 2011
File size: 117.5 kB
Specification Sheets
Brochures & Specifications: Take the Direct Route to DNA Sequencing
File size: 352.9 kB
Certificates
Certificates
A lot number is required to show results for certificates. To find your lot number on previous orders use our order status area.
5 results found
Lot Number | Certificate Type | Date | Product Code |
---|---|---|---|
2850384 | Certificate of Analysis | 31/01/2025 | 4458688 |
2807246 | Certificate of Analysis | 29/01/2025 | 4458688 |
2807231 | Certificate of Analysis | 29/01/2025 | 4458688 |
2807201 | Certificate of Analysis | 29/01/2025 | 4458688 |
2807238 | Certificate of Analysis | 29/01/2025 | 4458688 |
For Research Use Only. Not for use in diagnostic procedures.
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