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Beschreibung
Thermo Scientific™ Blue Carrier Protein is a highly-soluble, mollusk-derived hemocyanin formulated in convenient package sizes and a complete kit for carbodiimide (EDC-mediated) hapten-carrier conjugation via amine and carboxyl groups.
Highlights:
Convenient packages – choose lyophilized Blue Carrier Protein or complete EDC conjugation kit
High-yield conjugation – each molecule of CCH contains hundreds of primary amines available for coupling haptens via EDC or NHS ester crosslinkers
Validated quality – the immunogenic carrier protein is specially purified to ensure uniform chemical structure and solubility for consistent performance
Superior solubility – increased solubility compared to KLH allows Blue Carrier Protein to be used in a broader range of conjugation protocols to accommodate unusual or poorly soluble haptens
Highly immunogenic – provides essentially the same level of immunogenicity and phylogenetic distance as keyhole limpet hemocyanin (KLH)
Properties of Blue Carrier Protein:
- Alternative names: Concholepas concholepas hemocyanin, CCH
- Molecular weight: Whole molecule: ∽8,000,000; Subunits: ∽400,000 and ∽350,000
- Absorption: maxima 280nm, 350nm and 558nm
- Extinction coefficient (Abs. 1mg/mL solution): 1.4 at 280nm; 0.35 at 346nm
- A280/A350 Ratio: ∽4.0
- Copper/protein ratio: ∽16 Cu2+/protein molecule
- Isoelectric point: 6.0
- Solubility: Soluble in aqueous buffers pH 7.0 to 8.0
- Stability: Stable in aqueous buffers (∽pH 7.2 without Ca2+ or Mg2+)
Includes:
Kit contains lyophilized Blue Carrier Protein (in MES buffer), EDC crosslinker, buffers and desalting columns to easily prepare ready-to-use immunogens for injection
Spezifikation
Spezifikation
| Innehåll och lagring | 4°C. Shipped on ice. |
| Kvantitet | 100 mg |
| Produktlinje | Imject |
| Produkttyp | Imject Blue Carrier Protein |
For Research Use Only. Not for use in diagnostic procedures.
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