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Invitrogen™ eBioscience™ BrdU Kit for IHC/ICC Colorimetric
Contains the necessary reagents and buffers for identifying and examining proliferating cells by immunohistochemical or immunocytochemical analysis. Optimized for IHC with both frozen and paraffin-embedded BrdU-labeled mouse intestine and ICC of BrdU-pulsed HeLa cells grown on culture slides.
Brand: Invitrogen™ 8800-6599-45
Additional Details : Weight : 0.23750kg
Alert:
Contains Sodium azide, formaldehyde, and hydrogen peroxide
Includes: BrdU Fixation Buffer
BrdU Antigen Retrieval Solution (10X)
BrdU Blocking Solution
Anti-BrdU Biotin (clone BU20A) : 10 μg
Avidin-HRP
3, 3'-Diaminobenzidine (DAB) Substrate Solution: Reagent A (DAB Chromogen), Reagent B (DAB Diluent), and Dropper Bottle (for storage and application of working DAB solution)
Description
- Cells are labeled in vitro or in vivo with 5-bromo-2′-deoxyuridine (BrdU), which is incorporated into DNA in place of thymidine during the S-phase of the cell cycle
- Following fixation and antigen-retrieval steps, cells or tissue sections are stained for BrdU incorporation and visualized using an Avidin-HRP and DAB substrate reaction
Specifications
| BrdU | |
| DAB | |
| eBioscience™ | |
| IHC/ICC BrdU Kit |
| Colorimetric | |
| HRP (Horseradish Peroxidase) Substrate | |
| ICC (Immunochemistry), IHC (Immunohistochemistry) | |
| All species |
For Research Use Only. Not for use in diagnostic procedures.