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Invitrogen™ BrdU Monoclonal Antibody (BU-1)

Produktkod. 10009169
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Kvantitet:
100 μL
Förpackningsstorlek:
100µL
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10009169 100 μL 100µL
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Produktkod. 10009169 Leverantör Invitrogen™ Leverantörsnummer MA3071
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Beskrivning

Mouse Monoclonal Antibody

MA3-071 detects 5-Bromo-2-deoxyuridine (BrdU) in many samples. MA3-071 has been successfully used in immunofluorescence, immunohistochemistry, and flow cytometry applications. The MA3-071 immunogen is 5-iodouridine covalently coupled to ovalbumin. This antibody was orginally validated as part of a Thermo Scientific Cellomics High Content Screening Kit. The antibody sold separately may have slightly different performance and may need to be further optimized for the best results.

Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is a synthetic nucleoside that is an analogue of thymidine. BrdU is commonly used in the detection of proliferating cells in living tissues, and can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. Antibodies specific for BrdU can then be used to detect the incorporated chemical, thus indicating cells that were actively replicating their DNA. Binding of the antibody requires denaturation of the DNA, usually by exposing the cells to acid or heat.
TRUSTED_SUSTAINABILITY

Specifikationer

Antigen BrdU
Användningsområden Flow Cytometry, Immunohistochemistry, Immunocytochemistry
Klassificering Monoclonal
Klona BU-1
Koncentration 0.05 mg/mL
Konjugera Unconjugated
Formulering 0.01M HEPES with 0.15M NaCl, 30% glycerol and 0.01% sodium azide
Gene Alias 5-Bromo-2-deoxyuridine; Bromodeoxyuridine
Värd art Mouse
Immunogen 5-iodouridine covalently coupled to ovalbumin.
Reningsmetod Protein A
Kvantitet 100 μL
Regulatorisk status RUO
Primär eller sekundär Primary
Målarter Chemical
Innehåll och lagring -20°C
Produkttyp Antibody
Form Liquid
Isotyp IgG2a
Målmolekyl Chemical
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