c-Met, clone: eBioclone 97, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 14-8858-82
Additional Details : Weight : 0.01000kg
DescriptionDescription: 97 monoclonal antibody was generated against a human c-Met-Ig fusion protein, and reacts with human c-Met (HGFR)-transfected cells. Human c-Met is a 145kDa receptor tyrosine kinase (RTK) expressed by epithelial cells of the brain, kidney, liver and other tissues. Binding of its ligand, Hepatocyte Growth Factor (HGF), triggers receptor autophosphorylation, and activation of several downstream effectors including the mitogen-activated protein kinases ERK-1 and ERK-2, and PLC gamma;. Activation of the c-Met signal transduction pathway leads to mulitple cellular responses including cell motility, scattering, proliferation, survival and angiogenesis. Mutations in human c-Met have been implicated in the development of several malignancies.Applications Reported: This eBioclone 97 antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBioclone 97 antibody has been tested by flow cytometric analysis of human c-Met-transfected cells. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.09% sodium azide; pH 7.2|
|AUTS9, HGFR, RCCP2, c-Met|
|IgG1, kappa, kappa|
|Store at 2-8°C.|
For Research Use Only.