c-Myc, clone: 9E10, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 14-6784-82
Additional Details : Weight : 0.01000kg
DescriptionDescription: The 9E10 antibody reacts with human c-Myc p67; the antibody was raised against a synthetic peptide corresponding to amino acids 408-439 within the carboxy terminal domain of human c-Myc. This antibody can be used to detect myc-tagged protein. The transcription factor c-Myc is a proto-oncogene that is at the focal point in cell cycle regulation, metabolism, apoptosis, differentiation, cell adhesion, and tumorigenesis. In normal cells the expression of c-Myc is tightly regulated but in human cancers c-Myc is frequently deregulated. C-Myc also plays a pivotal role in apoptosis, most notably its connections to the CD95/Fas death receptor pathway. These different biological responses to c-Myc are most likely the result of different overlapping subsets of c-Myc target genes.Applications Reported: This 9E10 antibody has been reported for use in immunoprecipitation, western blotting, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections.Applications Tested: This 9E10 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded tissue using low or high pH antigen retrieval and can be used at less than or equal to 5 ug/mL. This 9E10 antibody has also been tested by western blot and can be used 5 ug/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.09% sodium azide; pH 7.2|
|c Myc, Cellular myelocytomatosis oncogene, Myc protein, Myc proto oncogene protein, Myc2, Niard, Nird, v myc avian myelocytomatosis viral oncogene hom|
|Immunohistochemistry (Paraffin)þImmunoprecipitationþWestern Blot|
|Store at 2-8°C.|
For Research Use Only.