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Invitrogen™ eBioscience™ Calcein AM Viability Dye (UltraPure Grade)
A membrane-permeable live-cell labeling dye. Upon entering the cell, intracellular esterases cleave the acetoxymethyl (AM) ester group, yielding the membrane-impermeable Calcein fluorescent dye.
2510.00 SEK - 2585.00 SEK
Specifications
| Color | Green |
|---|---|
| Detection Method | Fluorescent |
| For Use With (Application) | Viability Assay |
| For Use With (Equipment) | Fluorescence Microscope, Flow Cytometer |
| Product Type | Dye |
| Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
|---|---|---|---|---|---|---|---|---|---|
| Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
|
15560597
|
Invitrogen™
65-0853-78 |
20 x 50 μg |
2510.00 SEK
Pack of 20 |
In Stock Log in to see stock. |
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|
15550597
|
Invitrogen™
65-0853-39 |
1 mg |
2585.00 SEK
1mg |
In Stock Log in to see stock. |
Please sign in to purchase this item. Need a web account? Register with us today! | ||||
Description
- Upon entering the cell, intracellular esterases cleave the acetoxymethyl (AM) ester group, yielding the membrane-impermeable Calcein fluorescent dye
- Apoptotic and dead cells with compromised cell membranes do not retain Calcein
- Calcein is optimally excited at 495 nm and has a peak emission of 515 nm
- For flow cytometric analysis, it can be excited off the blue laser line (488 nm) and detected using filters for FITC/Alexa Fluor™ 488 (530/30)
- For multicolor flow cytometry, concentrations in the range of 5 to 20 nM are recommended to ensure that compensation can be performed successfully
- Co-staining with Annexin V or 7-AAD is recommended to allow the greatest resolution between live and dead/apoptotic cells
- If single-color analysis is desired, improved resolution between live and dead/apoptotic cells may be obtained by using either Calcein Blue AM (cat. 65-0855) or Calcein Violet 450 AM (cat. 65-0854)
- Calcein AM should be reconstituted in high-quality, freshly opened DMSO
- Once reconstituted, it should be stored at -20°C with dessicant and used within a short period of time (avoid freeze-thawing)
- Purity: >95% (determined by HPLC)
Specifications
| Green | |
| Viability Assay | |
| Dye | |
| eBioscience™ |
| Fluorescent | |
| Fluorescence Microscope, Flow Cytometer | |
| 495/515 |
For Research Use Only. Not for use in diagnostic procedures.