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Thermo Scientific Chemicals CAPS, 0.5M buffer soln., pH 10.5 Product Code.: 15484819
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Thermo Scientific Chemicals CAPS, 0.5M buffer soln., pH 10.5

Product Code. 15484819
250 mL
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250 mL
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Product Code. 15484819

Brand: Thermo Scientific Chemicals J62446.AK

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Description

CAS: 1135-40-6 | C9H19NO3S | 221.32 g/mol

CAPS (N-cyclohexyl-3-aminopropanesulfonic acid) buffer salt is used to formulate CAPS buffer, a zwitterionic buffer that is useful in the range of pH 7.9-11.1. CAPS buffer is widely used in Western and immunoblotting experiments as well as protein sequencing and identification. Used in the electrotransfer of proteins to PVDF (sc-3723) or nitrocellulose membranes (sc-3718, sc-3724). The high pH of this buffer makes it useful for the transfer of proteins with a pI > 8.5. CAPS is not one of the original Good’s buffers, although it has a similar structure to the other propanesulfonic acids and was selected as a highly water soluble buffering reagent with an optimum buffering pH of 10.4 and minimal reactivity with enzymes or proteins, minimal salt effects.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

Applications
CAPS (N-cyclohexyl-3-aminopropanesulfonic acid) buffer salt is used to formulate CAPS buffer, a zwitterionic buffer that is useful in the range of pH 7.9-11.1. CAPS buffer is widely used in Western and immunoblotting experiments as well as protein sequencing and identification. Used in the electrotransfer of proteins to PVDF (sc-3723) or nitrocellulose membranes (sc-3718, sc-3724). The high pH of this buffer makes it useful for the transfer of proteins with a pI>8.5. CAPS is not one of the original Good’s buffers, although it has a similar structure to the other propanesulfonic acids and was selected as a highly water soluble buffering reagent with an optimum buffering pH of 10.4 and minimal reactivity with enzymes or proteins, minimal salt effects.

Solubility
Soluble in water (100 g/L) at 20°C, and methanol (1%).

Notes
Store at room temperature. Ensure good ventilation. Incompatible with oxidizing agents.
TRUSTED_SUSTAINABILITY

Chemical Identifiers

CAS 1135-40-6
Molecular Formula C9H19NO3S
Molecular Weight (g/mol) 221.32
MDL Number MFCD00003837
InChI Key PJWWRFATQTVXHA-UHFFFAOYSA-N
IUPAC Name 3-(cyclohexylamino)propane-1-sulfonic acid
SMILES OS(=O)(=O)CCCNC1CCCCC1

Specifications

Concentration 0.5 M
pH 10.5
Physical Form Liquid
Quantity 250 mL
Chemical Name or Material CAPS

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Lot Number Certificate Type Date Catalog Number
Lot NumberR26L515 Certificate TypeCertificate of Analysis Date22/05/2025 Catalog NumberJ62446.AK,J62446.AP
Lot NumberN14L559 Certificate TypeCertificate of Analysis Date14/02/2025 Catalog NumberJ62446.AK,J62446.AP
Lot NumberM31L540 Certificate TypeCertificate of Analysis Date31/01/2025 Catalog NumberJ62446.AK,J62446.AP
Lot NumberM16L504 Certificate TypeCertificate of Analysis Date18/01/2025 Catalog NumberJ62446.AK,J62446.AP
Lot NumberY18K561 Certificate TypeCertificate of Analysis Date26/11/2024 Catalog NumberJ62446.AK,J62446.AP

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