Caspase 1, clone: 5B10, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 14-9832-80
Additional Details : Weight : 0.01000kg
DescriptionDescription: The monoclonal antibody 5B10 recognizes mouse Caspase-1, a member of the cysteine-requiring aspartate protease family, which plays a critical role in cell suicide known as apoptosis. In particular, it converts pro-IL-1beta to mature IL-1beta; hence it is also called ICE (interleukin-1beta converting enzyme). Upon activation Caspase-1 exists as a tetramer of two p20 and two p10 subunits. There are several isoforms of Caspase-1. The 5B10 antibody recognizes both the pro-form of about 42kDa and the active p20 form.Applications Reported: This 5B10 antibody has been reported for use in immunoblotting (WB).Applications Tested: This 5B10 antibody has been tested by western blot analysis of lysates prepared from the J774 cell line. This can be used at 1-5 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.09% sodium azide; pH 7.2|
|ICE, IL1BC, P45|
|Store at 2-8°C.|
For Research Use Only.