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CCL2 (MCP-1), PE, clone: 5D3-F7, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 12-7099-80

Additional Details : Weight : 0.09500kg

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Product Code. 15517096

  • 850.00 SEK / 25µg


EU Stock 1
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Disclaimers:

For Research Use Only.


Description

Description

Description: The 5D3-F7 antibody reacts with human, cynomolgus, and rhesus monkey monocyte chemoattractant protein-1 (MCP-1), also known as monocyte chemotactic and activating factor (MCAF) and CCL2. MCP-1 is a CC chemokine which presents as a dimer. Derived from monocytes and vascular endothelial cells, it exerts chemoattractant activity on a variety of cell types, such as monocytes, basophils, T cells and NK cells via chemokine receptors such as CCR2, CCR5, and CCR10.Applications Reported: This 5D3-F7 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This 5D3-F7 antibody has been tested by intracellular staining and flow cytometric analysis of stimulated normal human peripheral blood cells using the Intracellular Fixation & Permeabilization Buffer Set (cat. 88-8824) and protocol. Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online. This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CCL2 (MCP-1)
5D3-F7
PE
Conjugated
Murine
25μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Monoclonal
Flow Cytometry (Intracellular Staining)
0.2mg/mL
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
MCAF
IgG1
RUO
Primary
Human
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