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CCL3 (MIP-1 alpha) Rat anti-Mouse, PerCP-eFluor™ 710, Clone: DNT3CC, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 46-7532-80

Additional Details : Weight : 0.09500kg

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Product Code. 15559366

  • 1214.00 SEK / 25µg


Estimated Shipment
14-12-2020
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Description

Description

A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser. Filtration: 0.2 μm post-manufacturing filtered. Macrophage inflammatory protein-1a (MIP-1a) is an 8kDa member of the C-C chemokine subfamily. C-C chemokines are chemoattractants and activators for monocytes and T cells. MIP-1a has been identified as one of the major HIV-SFs produced by CD8+ T cells, along with MIP-1b and RANTES.
Specifications

Specifications

CCL3 (MIP-1 alpha)
Monoclonal
0.2 mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P10855
RP23-320E6.7, AI323804, G0S19-1, LD78alpha, MIP-1alpha, MIP1-(a), MIP1-alpha, Mip1a, Scya3
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Mouse
Flow Cytometry
DNT3CC
PerCP-eFluor™ 710
Ccl3
Liquid
Ccl3
IgG2a, kappa
25 μg
4° C, store in dark, DO NOT FREEZE!
Primary
20302
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