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CD101, clone: BB27, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-1019-82

Additional Details : Weight : 0.01000kg

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Product Code. 15247137

  • 1692.00 SEK / 100µg


Estimated Shipment date
from Supplier 25-09-2018
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Description

Description

Description: The monoclonal antibody BB27 reacts to human CD101 also known as V7 antigen, a member of the Ig superfamily. CD101 is a disulfide linked homodimer of unknown function. Expression of CD101 is found on monocytes, granulocytes and dendritic cells (Langerhan-like cells HLA-DR, CD1a, CD1c). In addition expression on T lymphocytes is important for cell activation. CD101+ CD28+ cells are very responsive to CD28 signaling. In combination with anti-CD28 or suboptimal levels of anti-CD3, anti-CD101 can increase proliferation thereby suggesting an activating role. The monoclonal antibody BB27 has been shown to inhibit the T cell reactivity in allogeneic and antigen-specific mixed DC-T cell cultures. Recently it has been demonstrated that mouse CD101 is found on a subpopulation of regulatory T cells (CD4, CD25, Foxp3 positive) that have high suppressor activity. Expression of CD101 on human PBMCs shows staining of about 30% of the Foxp3 positive cells. Studies have not confirmed higher suppressor activity in the human CD101 population.Applications Reported: This BB27 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining(frozen tissue).Applications Tested: This BB27 antibody has been tested by flow cytometric analysis of human peripheral blood cells. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD101
Monoclonal
0.5mg/mL
PBS with 0.09% sodium azide; pH 7.2
Q93033
RP11-27K13.2, IGSF2, V7
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow CytometryþImmunohistochemistry (Frozen)þImmunoprecipitation
BB27
Unconjugated
CD101
Liquid
CD101
IgG1
100μg
4° C
Primary
9398
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