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CD101 Rat anti-Mouse, PE, Clone: Moushi101, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 12-1011-80

Additional Details : Weight : 0.25000kg

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Product Code. 15238629

  • 898.00 SEK / 25µg

In stock, ready to ship: 1

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Description

Description

Description: The monoclonal antibody moushi101 recognizes mouse CD101, also known as IGSF2 and V7 (7 IgV domains). CD101 is a transmembrane glycoprotein containing 7 Ig-like IgV domains and a short cytoplasmic tail with several phosphorylation sites. Cross-linking CD101 can antagonize TCR/CD3-mediated lymphocyte activation. CD101 is expressed on monocytes, granulocytes, dendritic cells, Langerhans cells and activated T cells. In addition, CD101 is found on a subset of CD25+Foxp3+ T regulatory cells that showed higher suppressive properties in vitro and in vivo. Applications Reported: This Moushi101 antibody has been reported for use in flow cytometric analysis. Applications Tested: This Moushi101 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 μm post-manufacturing filtered.

CD101 also known as V7 antigen, a member of the Ig superfamily. CD101 is a disulfide linked homodimer of unknown function. Expression of CD101 is found on monocytes, granulocytes and dendritic cells (Langerhan-like cells HLA-DR, CD1a, CD1c). In addition expression on T lymphocytes is important for cell activation. CD101+ CD28+ cells are very responsive to CD28 signaling. In combination with anti-CD28 or suboptimal levels of anti-CD3, anti-CD101 can increase proliferation thereby suggesting an activating role. The monoclonal antibody BB27 has been shown to inhibit the T cell reactivity in allogeneic and antigen-specific mixed DC-T cell cultures. Recently it has been demonstrated that mouse CD101 is found on a subpopulation of regulatory T cells (CD4, CD25, Foxp3 positive) that have high suppressor activity. Expression of CD101 on human PBMCs shows staining of about 30% of the Foxp3 positive cells. Studies have not confirmed higher suppressor activity in the human CD101 population.
Specifications

Specifications

CD101
Monoclonal
0.2 mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
A8E0Y8
RP11-27K13.2, IGSF2, V7
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Mouse
Flow Cytometry
Moushi101
PE
Cd101
Liquid
Cd101
IgG2a, kappa
25 μg
4° C, store in dark, DO NOT FREEZE!
Primary
630146
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For Research Use Only.