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CD104 (Integrin beta 4), clone: 439-9B, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-1049-82

Additional Details : Weight : 0.01000kg

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Product Code. 15277147

  • 930.00 SEK / 100µg


EU Stock 1
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Description

Description

Description: The monoclonal antibody 439-9B recognizes human CD104, also known as integrin beta 4. CD104 is a 202kDa subunit that associates with integrin alpha 6 (CD49f), thereby forming the laminin receptor. Multiple splice variants have been identified. Expression is found on epithelial cells and tumor cells and has been shown to be involved in cell-matrix and cell-cell interactions. The role of CD104 in tumorigenicity is well established. The monoclonal antibody 439-9B has been reported to have no effect on adhesion of human colon adenocarcinoma cells. Moreover, this antibody crossreacts to bovine.Applications Reported: This 439-9B antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting under nonreducing conditions only, immunohistology staining of frozen tissue sections, and immunohistology staining of paraffin embedded tissue sections.Applications Tested: This 439-9B antibody has been tested by flow cytometric analysis of A549 cell line. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD104 (Integrin beta 4)
Monoclonal
0.5mg/mL
PBS with 0.09% sodium azide; pH 7.2
P16144
CD104 antigen, GP150, integrin beta-4, integrin beta-4 subunit, ITGB4
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
439-9B
Unconjugated
ITGB4
Liquid
ITGB4
IgG2b
100μg
Store at 2-8°C.
Primary
3691
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