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CD107a (LAMP-1), clone: eBio1D4B (1D4B), eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-1071-85

Additional Details : Weight : 0.01000kg

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Product Code. 15237167

  • 1944.00 SEK / 500µg


Estimated Shipment date
from Supplier 25-10-2018
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Description

Description

Description: The eBio1D4B monoclonal antibody reacts with mouse CD107a, also known as lysosomal-associated membrane protein-1 (LAMP-1). CD107a is a type I, lysosomal membrane protein that is extensively glycosylated. It is expressed constitutively in the late endosomes-lysosomes in all cells. CD107a is also transiently expressed on the cell surface of degranulating cytolytic T cells. Additionally, CD107a has been implicated in a variety of cellular functions including cancer metastasis and is also a marker for lysosomal storage disorders.Applications Reported: This eBio1D4B (1D4B) antibody has been reported for use in intracellular staining followed by flow cytometric analysis, immunoprecipitation, western blotting, immunohistochemical staining of frozen tissue sections, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections. (Fluorochrome conjugated eBio1D4B (1D4B) is recommended for use in intracellular flow cytometry.).Applications Tested: This eBio1D4B (1D4B) antibody has been tested by intracellular staining and flow cytometric analysis of mouse thioglycolate-elicited peritoneal exudate cells. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD107a (LAMP-1)
Monoclonal
0.5mg/mL
PBS with 0.09% sodium azide; pH 7.2
P11438
CD107a, LAMPA, LGP120, CD107 antigen-like family member A, LAMP-1, lysosome-associated membrane glycoprotein 1, lysosome-associated membrane protein 1
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow Cytometry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
eBio1D4B (1D4B)
Unconjugated
Lamp1
Liquid
Lamp1
IgG2a, kappa, kappa
500μg
Store at 2-8°C.
Primary
16783
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