CD107b (LAMP-2), clone: eBioABL-93 (ABL-93), eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 14-1072-82
Additional Details : Weight : 0.01000kg
DescriptionDescription: The eBioABL-93 monoclonal antibody reacts with mouse CD107b, also known as lysosomal-associated membrane protein-2 (LAMP-2). CD107b is a highly glycosylated protein of approximately 120kDa that is expressed intracellularly in lysosomal membranes. It is also transiently expressed on the surface of cytolytic T cells during degranulation, but to a lesser extent than CD107a. It has been reported that CD107b may be identical to the mouse Mac-3 antigen.Applications Reported: This eBioABL-93 (ABL-93) antibody has been reported for use in intracellular staining followed by flow cytometric analysis, immunoprecipitation, and immunohistochemical staining. It is also reported for use in surface staining in a flow cytometric based degranulation assay. (Fluorochrome conjugated eBioABL-93 (ABL-93) is recommended for use in intracellular flow cytometry.).Applications Tested: This eBioABL-93 (ABL-93) antibody has been tested by intracellular staining and flow cytometric analysis of mouse thioglycolate-elicited peritoneal exudate cells. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.09% sodium azide; pH 7.2|
|RP23-193O17.2, CD107b, Lamp-2, Lamp-2a, Lamp-2b, Lamp-2c, Mac3|
|Flow Cytometry, Immunohistochemistry, Immunoprecipitation|
|IgG2a, kappa, kappa|
|Store at 2-8°C.|
For Research Use Only.