CD11a (LFA-1alpha), clone: M17/4, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 14-0111-81
Additional Details : Weight : 0.01000kg
DescriptionDescription: The M17/4 monoclonal antibody reacts with mouse CD11a, the 180kDa integrin alpha L, also known as the lymphocyte function associated antigen-1 (LFA-1) alpha chain. LFA-1, formed by non-covalent association of CD11a with CD18 (integrin beta 2), serves as an important adhesion molecule involved in lymphocyte and granulocyte function. CD54 (ICAM-1), CD102 (ICAM-2), and CD50 (ICAM-3) are ligands for LFA-1. CD11a is expressed by all leukocytes.Applications Reported: The M17/4 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining. M17/4 has also been reported in blocking of CD11a function. (Please use Functional Grade purified M17/4, cat. 16-0111, in functional assays.).Applications Tested: The M17/4 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.09% sodium azide; pH 7.2|
|CD11 antigen-like family member A, LFA-1 alpha, LFA-1A, antigen CD11A p180, lymphocyte function-associated antigen 1 alpha polypeptide, integrin alpha-L|
|Flow CytometryþFunctional AssayþImmunohistochemistryþImmunoprecipitationþNeutralization|
|IgG2a, kappa, kappa|
|Store at 2-8°C.|
For Research Use Only.