missing translation for 'onlineSavingsMsg'
Learn More

CD11a (LFA-1alpha) Monoclonal Antibody (M17/4), Functional Grade, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

752.00 SEK - 3110.00 SEK

Specifications

Antigen CD11a (LFA-1alpha)
Clone M17/4
Concentration 1 mg/mL
Applications Control, Flow Cytometry, Functional Assay
Classification Monoclonal
View More Specs

Products 3
Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
15218377
missing translation for 'viewDocuments' Promotion Details
Affymetrix eBioscience
16-0111-82
100 μg
1660.00 SEK
100µg
View alternative products
missing translation for 'toSaveUpto' 
»
Estimated Shipment: 08-05-2024
to see stock.
Please to purchase this item. Need a web account? Register with us today!
15228377
missing translation for 'viewDocuments' Promotion Details
Affymetrix eBioscience
16-0111-85
500 μg
3110.00 SEK
500µg
View alternative products
missing translation for 'toSaveUpto' 
»
Estimated Shipment: 08-05-2024
to see stock.
Please to purchase this item. Need a web account? Register with us today!
15208377
missing translation for 'viewDocuments' Promotion Details
Affymetrix eBioscience
16-0111-81
50 μg This item is currently unavailable or has been discontinued.
View the product page for possible alternatives.
N/A View alternative products
missing translation for 'toSaveUpto' 
»
Description

Description

Description: The M17/4 monoclonal antibody reacts with mouse CD11a, the 180 kDa integrin alpha L, also known as the lymphocyte function associated antigen-1 (LFA-1) alpha chain. LFA-1, formed by non-covalent association of CD11a with CD18 (integrin beta 2), serves as an important adhesion molecule involved in lymphocyte and granulocyte function. CD54 (ICAM-1), CD102 (ICAM-2), and CD50 (ICAM-3) are ligands for LFA-1. CD11a is expressed by all leukocytes. Applications Reported: The M17/4 antibody has been reported for use in flow cytometric analysis. M17/4 has also been reported in blocking of CD11a function. Applications Tested: The M17/4 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Storage and handling: Use in a sterile environment. Filtration: 0.2 μm post-manufacturing filtered. Purity: Greater than 90%, as determined by SDS-PAGE. Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay. Aggregation: Less than 10%, as determined by HPLC.

CD11a (LFA-1 alpha, ITGAL) together with CD18 constitute leukocyte function-associated antigen 1 (LFA-1), the alphaLbeta2 integrin. CD11a is implicated in activation of LFA-1 complex. CD11a plays a central role in leukocyte intercellular adhesion through interactions with its ligands, ICAMs 1-3 (intercellular adhesion molecules 1 through 3), and also functions in lymphocyte costimulatory signaling. CD11a is expressed on the plasma membrane of leukocytes in a low-affinity conformation. Cell stimulation by chemokines or other signals leads to induction the high-affinity conformation, which supports tight binding of CD11a to its ligands, the intercellular adhesion molecules ICAM-1, -2, -3. CD11a is thus involved in interaction of various immune cells and in their tissue-specific settlement, but participates also in control of cell differentiation and proliferation and of T-cell effector functions. Blocking of CD11a function by specific antibodies or small molecules has become an important therapeutic approach in treatment of multiple inflammatory diseases. For example, humanized anti- CD11a antibody Efalizumab (Raptiva) is being used to interfere with T cell migration to sites of inflammation, binding of cholesterol-lowering drug simvastatin to CD11a allosteric site leads to immunomodulation and increase in lymphocytic cholinergic activity. Two transcript variants encoding different isoforms of CD11a have been found. Diseases associated with CD11a dysfunction include benign cephalic histiocytosis and leukocyte adhesion deficiency.
Specifications

Specifications

CD11a (LFA-1alpha)
1 mg/mL
Monoclonal
Liquid
RUO
PBS with no preservative; pH 7.2
(p180); alpha L integrin; antigen CD11A (p180); antigen CD11A (p180), lymphocyte function-associated antigen 1, alpha polypeptide; antigen CD11A p180; CD11 antigen-like family member A; CD11a; integrin alpha L; integrin alphaL; integrin alpha-L; integrin alpha-L precursor; integrin gene promoter; integrin subunit alpha L; integrin surface receptor; integrin, alpha L; integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide); ITGAL; Leukocyte adhesion glycoprotein LFA-1 alpha chain; leukocyte function-associated molecule 1 alpha chain; LFA-1; LFA-1 alpha; LFA1A; LFA-1A; Ly-15; Ly-21; lymphocyte antigen 15; lymphocyte function associated antigen 1, alpha polypeptide; lymphocyte function-associated antigen 1; lymphocyte function-associated antigen 1, alpha polypeptide; lymphocyte function-associated antigen-1
ITGAL
Primary
4° C
ITGAL
M17/4
Control, Flow Cytometry, Functional Assay
Functional Grade
Rat
Mouse
P24063
16408
IgG2a κ
Affinity chromatography
Antibody
Videos
SDS
Documents

Documents

Certificates

For Research Use Only.