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CD127, clone: A7R34, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-1271-82

Additional Details : Weight : 0.01000kg

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Product Code. 15277197

  • 1278.00 SEK / 100µg


Estimated Shipment date
from Supplier 24-09-2018
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Description

Description

Description: The A7R34 monoclonal antibody reacts with mouse CD127, the high affinity alpha subunit of the mouse IL-7 receptor. IL-7 receptor alpha chain is expressed by immature B cells in the bone marrow, double-negative (CD4-CD8-), single-positive (CD4+ and CD8+), but not double-positive (CD4+CD8+) thymocytes. In the periphery, mature T cells express CD127 at low level. A7R34 inhibits binding of IL-7 to its receptor and has been used in in vivo and in vitro studies to elucidate the role of IL-7 in T and B cell development and activation. Binding of A7R34 blocks the binding of SB/199, another antibody which recognizes mouse CD127.Applications Reported: This A7R34 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistology staining of frozen tissue sections. It has also been reported in blocking of IL-7 binding in functional studies. (Please use Functional Grade purified A7R34, cat. 16-1271, in functional assays.).Applications Tested: The A7R34 antibody has been tested by flow cytometric analysis of mouse thymocytes and splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD127
Monoclonal
0.5mg/mL
PBS with 0.09% sodium azide; pH 7.2
P16872
CD127, IL-7Ralpha, MGC107557
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow CytometryþFunctional AssayþImmunohistochemistry (Frozen)þImmunoprecipitationþNeutralization
A7R34
Unconjugated
Il7r
Liquid
Il7r
IgG2a, kappa, kappa
100μg
4° C
Primary
16197
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