CD127, PE, clone: A7R34, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 12-1271-83
Additional Details : Weight : 0.25000kg
DescriptionDescription: The A7R34 monoclonal antibody reacts with mouse CD127, the high affinity alpha subunit of the mouse IL-7 receptor. IL-7 receptor alpha chain is expressed by immature B cells in the bone marrow, double-negative (CD4-CD8-), single-positive (CD4+ and CD8+), but not double-positive (CD4+CD8+) thymocytes. In the periphery, mature T cells express CD127 at low level. A7R34 inhibits binding of IL-7 to its receptor and has been used in in vivo and in vitro studies to elucidate the role of IL-7 in T and B cell development and activation. Binding of A7R34 blocks the binding of SB/199, another antibody which recognizes mouse CD127.Applications Reported: The A7R34 antibody has been reported for use in flow cytometric analysis.Applications Tested: The A7R34 antibody has been tested by flow cytometric analysis of mouse thymocytes and splenocytes. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|CD127, IL-7Ralpha, MGC107557|
|IgG2a, kappa, kappa|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
For Research Use Only.