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CD13, PerCP-eFluor™ 710, clone: WM-15 (WM15), eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 46-0138-42

Additional Details : Weight : 0.23750kg

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Product Code. 15509086

  • 2625.00 SEK / 100 tests


Estimated Shipment date
from Supplier 25-07-2018
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Description

Description

Description: The WM-15 monoclonal antibody reacts with human CD13, also known as aminopeptidase N. CD13 is a transmembrane ectoenzyme occurring on monocytes and granulocytes but not lymphocytes in blood. It is also reportedly on a wide variety of cells and in a wide variety of tissues, including endothelial cells, epithelial cells, fibroblasts, kidney proximal tubules, intestine, and placenta. CD13 is reported to have many different functions including degradation of enkephalins and endorphins, terminal degradation of peptides, amino acid scavenging, antigen processing, and adhesion and migration of cells.Applications Reported: This WM-15 (WM15) antibody has been reported for use in flow cytometric analysis.Applications Tested: This WM-15 (WM15) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 0.25 μL (5 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD13
Monoclonal
5μl/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
P15144
AP-M, AP-N, Apn, Cd13, P150, alanyl aminopeptidase, aminopeptidase M, aminopeptidase N, aminopeptidase N/CD13, membrane protein p161, microsomal aminopeptidase
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry
WM-15 (WM15)
PerCP-eFluor 710
ANPEP
Conjugated
ANPEP
IgG1, kappa
100 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
290
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